Zhou Bin and his team work together to reveal the cell fate and function of pericardial macrophages in cardiac inflammation and repair

Cardiovascular disease has always been an important cause of death worldwide

Recently, Paul Kubes' research group found that Gata6 ⁺ macrophageslocated in the pericardial cavity play an important role in structural cardiac remodeling and myocardial infarction (MI, Myocardial infarction) repair in mice

The researchers of Zhou Bin's group first proved that the newly established dual-homologous recombinase-mediated genetic lineage technology ( CD45-Dre; Gata6-iCreER; R26-tdTomato tool mouse) can be efficiently Specific labeling of macrophages in the pericardial cavity

To further elucidate the function of pericardial macrophages, the researchers constructed a conditional knockout of mouse CD45 -Dre; Gata6-iCreER; R26-tdTomato/iDTR and Gata6 for diphtheria toxin-mediated depletion of pericardial macrophages in mice -Dre;Gata6-iCreER/flox;R26-tdTomato

In addition, in order to exclude the differences produced by the experimental model, the researchers also constructed a bone marrow transplantation model to further clarify the function of pericardial macrophages in cardiac injury

In conclusion, the researchers used the established dual-homologous recombinase-mediated lineage tracing technique to specifically label cardiac macrophages, a subset of pericardial macrophages

Jin Hengwei, a postdoctoral fellow of Zhou Bin's research group at the Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, and Liu Kuo, a postdoctoral fellow at the Hangzhou Institute for Advanced Study, Chinese Academy of Sciences, are the co-first authors of the paper.

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　　Macrophages free in the pericardial cavity will accumulate in the thickened epicardial mesothelial layer in response to the inflammatory response when the heart is injured, but cannot penetrate into the myocardium to participate in the repair of the injury