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Zhongke Xinsheng's new product ultra-trace proteomics facilitates clinical sample research

 Last Update: 2022-08-30
 Source: Internet
 Author: User

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With the shocking launch of single-cell proteomics products in 2021, the protein identification of single cells has been achieved and a major breakthrough in sorting 1,000 cells at one time has been completed.

So what are the features of the microproteomics launched this time, and what are the differences between it and the classical proteomics method and single-cell proteomics method?

Let's follow the editor to unravel the mystery of microproteomics from three aspects: sample range, method characteristics and identification depth

Very small sample volume as low as 100 cells, tissue volume as low as 1mg, and body fluids as low as 5μL

According to the sample types and their characteristics, we can classify them into three categories (Figure 1).

That's right, our microproteomics enables detection of these sample types

One-step extraction of protein peptides + a new generation of 4D-DIA platform = a new ultra-trace solution

Due to the small amount of samples such as flow sorting cells and body fluids, if traditional proteomics methods are used for processing, not only the sample size will be lost, but the detection rate of low-abundance proteins will also be greatly affected

·Protein peptide one-step extraction method

The single-pot and miniaturized in-solution digestion (SMID) method and SP3 and other protein extraction methods suitable for very small samples are used for omics preparation before the machine, and at the same time, complex intermediate processes such as protein quantification, electrophoresis, and tube transfer are eliminated.

·Mass spectrometry acquisition method optimization

If a worker wants to do a good job, he must first sharpen his tools

Figure 3 Experiment process of ultra-trace proteomics

100 cells 1500+, 1000 cells 5000+ identification capabilities, compatible with a variety of cell types, suitable for stem cells, reproduction, immunity and other fields

In addition to the characteristic advantages of reducing sample usage, microproteomics has also achieved great breakthroughs in the depth of protein identification

In addition, new breakthroughs in identification depth were also achieved in different sample types: the identification number of 1 egg cell was as high as 2300, and the identification number of 1000 flow-sorted cells was as high as 5100 (Figure 5)

On the occasion of the launch of new products, all teachers who place an order can enjoy the fresh tasting price.

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