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Yeast Two-Hybrid Approaches to Dissecting the Plant Defense Response

 Last Update: 2021-01-13
 Source: Internet
 Author: User

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We describe a reliable GAL4-based yeast two-hybrid system for identifying and isolating clones encoding proteins interacting with a protein of interest. This two-hybrid system gives extremely low background and few false-positive clones, making it ideal for library screening purposes. We have successfully used it not only to isolate
Arabidopsis
NPR1-interactors from rice but also to pull out the rice NPR1 ortholog using one of the interactors as bait.

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