 Home > Biochemistry News > Plant Extracts News > Yeast ProteinProtein Interaction Assays and Screens

Yeast ProteinProtein Interaction Assays and Screens

 Last Update: 2020-11-10
 Source: Internet
 Author: User

Tags

library

transcription factor proteins

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Most transcription factors fulfill their role in protein complexes. As a consequence, information about their interaction capacity sheds light on a protein’s function and the molecular mechanism underlying this activity. The yeast two-hybrid GAL4 (Y2H) assay is a powerful method to unravel and identify the composition of protein complexes. This in vivo based system makes use of two functional protein domains of the GAL4 transcription factor, each fused to a protein of interest. Upon interaction between the two proteins under study, a transcriptional activator gets reconstituted and reporter genes get activated, allowing the yeast to grow on selective medium. In this chapter protocols are given for Y2H library screening, directed Y2H screening, Y2H matrix screening, and Y
n
H screening involving more than two proteins.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Monobenzone CAS 103-16-2 White Crystal Powder USP/BP Standard

CAS No.: 103-16-2

Cosmetics Grade

$1-1.2/KG EXW

Carob Extract

CAS No.: 84961-45-5

pharmaceutical grade/food grade

$10-13/KG FOB

Premium ingredient Panax Ginseng Extract 80%

CAS No.: 50647-08-0

Food Grade

$25-30/KG FOB

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

single use technology(195)

announcements today(81)

ups united states(221)

united states logo(227)

80 20 company(94)

healthy food name(42)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.