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Tissue culture is an important plant vegetative propagation technology, and it is also the basis for the application of modern agricultural molecular breeding technologies such as gene editing
"The organ regeneration capacity of callus" has always been one of the core scientific issues in the field of plant regeneration.
The author first performed single-cell sequencing on the callus produced from the hypocotyl of Arabidopsis thaliana and confirmed that the callus is similar to the root primordium or root tip meristem and can be roughly divided into three layers: the outer layer of cells is similar to the root tip.
Based on the above results, the author speculates on the principle that callus has the ability to regenerate organs
Doctoral student Zhai Ning of the Center for Excellence in Molecular Plant Science of the Chinese Academy of Sciences is the first author of the paper, and researcher Xu Lin is the corresponding author
Link to the paper: https:// align="center">
Callus has the ability to regenerate shoots and roots
The cells in the middle layer of callus are pluripotent
The principle that callus has the ability of organ regeneration