Which gases are used in anaerobic incubators

1.
Place accessories and appliances according to the requirements of use; 2.
Turn on the power to turn on the light, open the temperature controller, adjust the required temperature; 3.
Put 1000g of palladium granules (blocked) and 500g of desiccant in the operating room, and put in the Meilan indicator paper (closed); 4.
Close the indoor and outdoor doors of the sampling and vacuum calibration; 5.
Secondary displacement (nitrogen displacement) in the operating room: A.
Put the latex gloves on the flange ring of the observation plate and tie it tightly B.
Connect the nitrogen intake path, open the nitrogen control valve 1, make the gloves bulge, close the valve 1, and then tighten the bag mouth; 6.
The operation room D secondary displacement (nitrogen displacement) repeats a nitrogen filling process, the sampling chamber is first evacuated, and pay attention to opening and closing the exhaust
with the foot switch.

So three consecutive nitrogen displacements
.

7.
Operation chamber D four times displacement (mixed gas displacement):( mixture gas ratio is N285%, H210%, CO25% A.
The ventilation circuit opens the mixture valve 3 intake, the sampling chamber is first vacuumed when inflated, and the foot switch is used to open and close the exhaust; B.
Turn off the mixed gas valve 3, and open the valve 5, so that the mixed gas passes through the flow meter, adjust the flow meter, the flow rate is about 10ml per minute; C.
The mixed gas repeats 2-3 conversions, and after the above process, an anaerobic environment
is basically formed.

8.
Open the deaerator in the operating room, connect the power supply of the deaeration catalyst for catalytic deaeration, open the Meilan indicator paper to observe its change after one hour, and do not change the color to achieve anaerobic environment in the operating room; 9.
Open the ultraviolet sterilization lamp, sterilize the room for sterilization, and the sterilization time is customized
according to the specific experiment.