What to look out for when using a used liquid chromatography mass spectrometer

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.

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Requirements for the use of second-hand liquid chromatography mass spectrometer: 1.
The mobile phase must be filtered with HPLC-level reagents to remove particulate impurities and other substances (using a membrane filtration of 0.
45 μm or finer).
2, mobile phase filtration to use ultrasonic degassing, after degassing should be restored to room temperature after use
.

3, can not use pure acetonitrile as a mobile phase, which will make the check valve stick and cause the pump not to enter the liquid
.

4.
When using buffer solution, immediately after making the sample, rinse the pipeline and column with deionized water for one hour, and then rinse with methanol (or methanol aqueous solution) for more than 40 minutes to fully wash ionized
.

For the outside of the plunger rod, it is also necessary to rinse more than
20 mL with deionized water after the sample is done.

5, a long time without instruments, should be removed from the column with a plug sealed for preservation, pay attention to the fact that the column can not be preserved with pure water, but should use the organic phase (such as methanol, etc.
), because pure water is easy to grow mold
.

6.
After each sample, the injector
should be cleaned with a solvent that dissolves the sample.

7.
C18 column cannot enter protein samples, blood samples, and biological samples
.