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Label: Domestic PCR plate sealing film BUNSEN Bunsen BUNSEN Bunsen PCR plate use and sealing film use method? Recommended PCR models: Roche LC 96, LC 480, Cobas z 480, Eppendorf eprealplex, Bio-Rad Opticon 2, Bio-Rad CFX96, ABI 7900 HT FAST, ABI 7500 FAST, ABI StepOne&Plus, ABI Veriti Fast, etc.
Plate Membrane? Plate Membrane is a transparent plate sealing film using gel, which is widely used in experiments with 96/384 well plates, such as: PCR, qPCR, ELISA, cell culture, long-term storage, automated workstation processing It will be used in almost all experiments
.
Its main function is to ensure that the sealing film is closely attached to the 96/384 well plate to prevent liquid evaporation
.
The correct way to apply the film: Remove a single sheet of sealing film or sealing aluminum foil from the ziplock bag and reseal the ziplock bag to maintain an enzyme-free environment
.
▪ With the backing side up, hold the cover film or cover foil
.
▪ Fold down the end label at the tangent of the backing
.
▪ If the product used is a cover film or foil for single-ended labels, partially remove the liner, then anchor the cover film or foil to the board so that it seals over the entire board, then proceed to remove the liner
.
This method eliminates curling and rollback that can occur with sealing film or foil
.
▪ If using a product with two end labels, peel off the center liner in a continuous smooth motion
.
Slowly peel off the liner to reduce curling
.
Be careful not to touch the adhesive surface of the film
.
▪ Grasp the white parts at both ends with both hands and lower the diaphragm onto the orifice plate
.
▪ Use a lamination plate to slowly scrape the sealing film or foil to seal it to the plate
.
This step should be performed at least twice horizontally and vertically
.
Applying sufficient force is essential to obtain a good seal
.
▪ Scrape the lamination plate along all outer edges of the orifice plate at least twice, making sure to apply firm and constant pressure
.
▪ Inspect the plate after sealing to verify that the film/foil is tightly bonded to the plate
.
There should be no wrinkles in the sealing film or foil, if wrinkles are observed, the plate is not properly sealed
.
▪ It is best to leave the sealed plate for at least 10 minutes before starting a PCR experiment, the adhesion of the sealing film will increase with time
.
▪ Transfer the plate to the thermal cycler and run the thermal cycler
.
The use of imported PCR plates and the use of sealing film? Bunsheng has always regarded quality control as the life of the enterprise, and pursued the continuous improvement of enterprise competitiveness
.
The company has always adhered to the corporate spirit of abiding by the law, being strict with oneself, being lenient, and daring to take responsibility as the standard, maintaining and expanding the market with excellent quality and excellent service, and meeting the needs of customers to the greatest extent.
.
Win-win with customers is our development goal
.
Bunsen! Your trusted partner
.
We would like to sincerely cooperate with you to create a better future
.
Plate Membrane? Plate Membrane is a transparent plate sealing film using gel, which is widely used in experiments with 96/384 well plates, such as: PCR, qPCR, ELISA, cell culture, long-term storage, automated workstation processing It will be used in almost all experiments
.
Its main function is to ensure that the sealing film is closely attached to the 96/384 well plate to prevent liquid evaporation
.
The correct way to apply the film: Remove a single sheet of sealing film or sealing aluminum foil from the ziplock bag and reseal the ziplock bag to maintain an enzyme-free environment
.
▪ With the backing side up, hold the cover film or cover foil
.
▪ Fold down the end label at the tangent of the backing
.
▪ If the product used is a cover film or foil for single-ended labels, partially remove the liner, then anchor the cover film or foil to the board so that it seals over the entire board, then proceed to remove the liner
.
This method eliminates curling and rollback that can occur with sealing film or foil
.
▪ If using a product with two end labels, peel off the center liner in a continuous smooth motion
.
Slowly peel off the liner to reduce curling
.
Be careful not to touch the adhesive surface of the film
.
▪ Grasp the white parts at both ends with both hands and lower the diaphragm onto the orifice plate
.
▪ Use a lamination plate to slowly scrape the sealing film or foil to seal it to the plate
.
This step should be performed at least twice horizontally and vertically
.
Applying sufficient force is essential to obtain a good seal
.
▪ Scrape the lamination plate along all outer edges of the orifice plate at least twice, making sure to apply firm and constant pressure
.
▪ Inspect the plate after sealing to verify that the film/foil is tightly bonded to the plate
.
There should be no wrinkles in the sealing film or foil, if wrinkles are observed, the plate is not properly sealed
.
▪ It is best to leave the sealed plate for at least 10 minutes before starting a PCR experiment, the adhesion of the sealing film will increase with time
.
▪ Transfer the plate to the thermal cycler and run the thermal cycler
.
The use of imported PCR plates and the use of sealing film? Bunsheng has always regarded quality control as the life of the enterprise, and pursued the continuous improvement of enterprise competitiveness
.
The company has always adhered to the corporate spirit of abiding by the law, being strict with oneself, being lenient, and daring to take responsibility as the standard, maintaining and expanding the market with excellent quality and excellent service, and meeting the needs of customers to the greatest extent.
.
Win-win with customers is our development goal
.
Bunsen! Your trusted partner
.
We would like to sincerely cooperate with you to create a better future
.
