What is the difference between a real-time PCR instrument and a gene amplification instrument?

What is the difference between a real-time PCR instrument and a gene amplification instrument? The real-time fluorescence quantitative PCR instrument is composed of a control system, a power supply system, an optoelectronic system, a module part, a thermal cover part, a housing part, and a random software.
It is suitable for all instruments based on reactions that can label the products during PCR product generation.

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The gene amplification instrument is also called the PCR instrument.
Many times, people think that the real-time fluorescence quantitative PCR instrument is the same as the gene amplification instrument, but is it really the same? What are the differences between them? The following will tell you in detail
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The fluorescence quantitative PCR instrument has more fluorescence signal acquisition system and computer analysis and processing system than the gene amplification instrument (ordinary PCR instrument).
The real-time fluorescence quantitative PCR instrument is mainly used to quantitatively analyze and determine the gene transcription level, while the ordinary PCR instrument It is to do qualitative analysis and amplify gene fragments.
Quantitative PCR instruments can do the work of ordinary PCR instruments, but not vice versa.
Moreover, the cost is too high, and ordinary laboratories are not allowed to do so
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In short, the functions of the two cannot replace each other
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Ordinary gene amplification instrument (PCR instrument) can only qualitatively analyze the existence of target fragments
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But the price is much lower, and the running cost is much lower
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However, the amplification results cannot be obtained directly, and electrophoresis detection is also required
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, molecular marker screening of varieties, and even personal identification can be completed by ordinary PCR machines
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　　However, some experiments that require quantification use real-time quantitative PCR
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For example, detecting the number of certain pathogens, the expression of specific genes (such as quantitative analysis of RNA combined with reverse transcription), the copy number of certain genes in the genome (used to use southern blot technology) and so on
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Fluorescence quantitative PCR generally does not require electrophoresis analysis of amplification products, and the operation is relatively simple, but the consumables are really expensive
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To put it simply, see if there is a common PCR machine, and see how much fluorescence quantitative PCR is used
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 The above is the relevant introduction about the difference between real-time fluorescence quantitative PCR instrument and gene amplification instrument.
I believe that everyone has a corresponding understanding of this after reading, and I hope it can help everyone
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