What do you know about the environmental requirements for cell culture media?

In the large-scale culture of animal cells, the choice of medium is a key factor for the growth, proliferation and differentiation of cells in vitr.
Cell culture medium is designed and developed to simulate the in vivo growth environment of cells and maintain the nutrients for cell survival and proliferation in vitr.
With the continuous improvement of the performance of cell culture medium and the innovation of cell culture equipment, many scientists can carry out cell culture work conveniently and systematicall.
The development and optimization of culture media not only need to meet the nutrients required for cell growth and high expression, but also consider cell viability, metabolism, product quality, manufacturability, regulatory, supply chain and IP requirement.
Animal cell culture methods include primary and subcultur.
Culture methods include adherence, suspension and immobilization method.
Let's follow the editor to understand the requirements of the cell culture environment!Sterile and non-toxic: A sterile and non-toxic operating environment is the premise to ensure the successful in vitro culture of animal cell.
In vitro cultured cells face the challenge of being infected by microorganisms or affected by their own progenitor.
Therefore, during in vitro cell culture, it is necessary to remove the waste generated by the cells in time to ensure that a sterile and non-toxic living environment is provided for the cells cultured in vitr.
Gas: The gas is mainly O2 and CO2.O2 can provide energy to cells, and CO2 is not only a substance required for cell proliferation, but also its own metabolite.
In addition, CO2 also regulates the pH of the cell culture mediu.
Temperature: Appropriate temperature can maintain the continuous and vigorous growth of cell.
If the temperature exceeds the appropriate temperature range, it will not only affect the normal generation of cells, damage cells, and even cause them to di.

Buffer environment: The function of the buffer environment is to provide a culture medium with a pH in the physiological environment of the cultured cells, provide water and inorganic salts, and maintain the normal metabolism of cell.
Cell culture medium: The medium is divided into two types: natural medium and synthetic mediu.
It is the direct environment for cell growth and reproduction, and provides the required nutrients for cell.
Natural culture medium is obtained by separation and extraction from animal body fluids or tissue.
Substances such as plasma, serum and lymph can be used as natural medi.
Animal cell culture mainly uses synthetic mediu.
The synthetic medium contains basic substances such as inorganic salts, carbohydrates, vitamins, amino acids, et.
required for cell growth, and an appropriate amount of serum will be added if there are special requirement.