What can and cannot be done when using a clean bench?

What to do 1.
Always minimize clutter
.

A decontamination bench is not a locker
.

The presence of debris impedes laminar flow around the work area
.

Plus, the mess makes cleaning work surfaces very difficult
.

2.
Always wash your hands and arms before entering
.

People are the most critical source of contamination, and touch is the most common method of introducing contamination into sterile products
.

Proper hand washing is very important because there are countless bacterial components in your fingers
.

Hand washing should not only be done before, but also after, on the bench
.

 　　3.
Objects should be arranged in such a way that the full benefits of laminar air flow are fully obtained
.

Properly arranging everything in a laminar flow bench provides a work area that should be flushed directly with clean air from a ULPA filter
.

 　　4.
Avoid spraying or spraying the solution on the HEPA/ULPA filter
.

Do not allow any items to come into contact with the HEPA/ULPA filter so as not to pollute the downstream air
.

 　　5.
Remove the outer bag and wrap around the edges of the work area
.

Outer bags and wrappers are non-sterile items, which means they can introduce contaminants into the bench
.

They are also considered chaotic, so they can disrupt laminar airflow patterns
.

 　　6.
Do not put large objects behind the hood
.

Large objects can contaminate everything else downstream and can also disrupt laminar airflow patterns, suspending contaminants and removing them from the area
.

 　　7.
Keep coughing, move quickly and speak as little as possible in the main work area
.

Human activity generates particles
.

Particles disrupt the laminar flow of air, which can also be a cause of pollution
.

If talking is necessary, keep it away from the workbench
.

 　　8.
Be sure to sterilize the material before entering the laminar flow cabinet
.

Only place sterile materials in a clean work area
.

Make sure all items are properly sanitized so as not to contaminate the bench
.

 　　NOT DO 1.
Put any waste and other items in the hood
.

Waste and non-sterile items can cause airflow turbulence and introduce contaminants into clean benches and sterile samples
.

 　　2.
Wear any jewelry on hands and wrists
.

Jewelry are non-sterile items, so they can introduce bacteria or particles into a clean work area
.

They also reduce hand cleanliness if worn on a bench
.

 　　3.
Keep critical items away from air sources
.

Maintain a direct open path between the filter and the interior area of ​​the cooker hood where the work program is carried out
.

The air downstream of non-sterile items is contaminated with particles produced by these items
.

 　　4.
When opening the ampoule or adjusting the syringe, aim near the filter
.

Salt solutions in ampoules and syringes can deposit on HEPA/ULPA filters, which can damage the filter
.

 　　5.
Bring unnecessary items to the main work area
.

The introduction of unnecessary items into the work area should be avoided as much as possible
.

Traffic flow into the clean area should be minimized
.

 　　6.
Place large objects near the rear of the cabinet as they can disrupt the laminar flow pattern of the air and contaminate everything downstream
.

7.
Use non-sterile materials
.

Unsterilized materials introduce contaminants into a clean bench, thus defeating the primary purpose of a decontamination bench, which is to protect your product from indoor contaminants
.