What are the two main points of using anaerobic incubators?

The use of the anaerobic incubator is actually 2 steps: the formation of anaerobic environment in the operation room and the insertion and culture of the strain, but each step requires a lot of small steps to complete, let's take a look at it
together.

First, the operation room anaerobic environment formation: according to the requirements of use, place the necessary accessories and appliances, and put two five-poison plastic bags
into the operation room.

After turning on the device, after the power switch, press the total power key on the control board to power on the device
.

First insert the rubber tube into the air inlet of the operating room, the other inserts a plastic bag, connects the nitrogen inlet path, opens the nitrogen control valve of the operation room of the anaerobic incubator, so that the two plastic bags are full of nitrogen, closes the solenoid valve, and then tightens the bag mouth, puts the latex gloves on the observation plate flange ring and tightens it, and gradually puts the nitrogen in the plastic bag in the operation room until all are removed
.

Open the palladium granular oxygenator in the operation room of the anaerobic incubator, turn on the power supply of the deaeration catalytic catalyst for catalytic deoxygenation, and drop a little clean water on the reaction area of the anaerobic indicator strip, after 1-2 hours, observe its condition, and the reaction part of the dark blue becomes light blue for the operation room to achieve an anaerobic environment
.

Second, the placement and culture of strains: check whether the door in the sampling room of the anaerobic incubator is closed, open the door outside the sampling room, close the outer door after putting the bacteria into the sampling room, fill the sampling room with nitrogen to replace the process three times, and then open the oxygen valve intake in the sampling room, so that the pointer returns to zero and closes the valve
.

Anaerobic incubator needs long-term continuous use of the conditions, every day in the operating room into the indicator bar observation, if not normal must be re-aerated, to long-term continuous input of trace amounts of mixed gas, so that the nitrogen energy and trace amount of nitrogen combined by catalytic absorption, to ensure the indoor anaerobic state, make up the mixture flow is selected to be about 10 ml per minute, continuous culture running for one day, replace the oxygen scavenger and desiccant
.