What are the advantages of cell culture bags compared to using culture flasks?

Cell culture bags are made of waterproof breathable membranes, and gases can pass through microporous membranes by the diffusion principle, and liquid water or water droplets cannot pass through
water molecules due to their surface tension.
Made of FEP material, it has a better cell proliferation effect than ordinary EVA material, and in the currently tested cell culture species, the cell proliferation effect is 20-40%
more than that of ordinary cell culture bags.
Product advantages: 1.
Thermal state: designed to be directly placed in
liquid nitrogen.
Flexibility can be maintained between -196°C and +200°C without significant loss of
strength.
2.
Inert: The inner layer adopts FEP and does not contain plasticizers or chemical residues, so the bag will not affect the material
stored inside.
Both the inner and outer layers are inert and do not contain any infiltratable or cocoa-carrotants
.
3.
Sealing tape: When it needs to be placed in the vapor state, a self-adhesive sealing tape (part code: CT)
can be optionally equipped.
When storage in a liquid state is required, a standard heat seal (550 °C)
is recommended.
The bag itself also has a screen-printed writing area
.
4.
Volume: The square seal mouth means that the bag has a larger internal volume
than the herringbone seal.
Easy-to-tear sealing tapes open
more easily than herringbone bags.
Compared with the use of culture flasks, cell culture bags can obtain a larger number of cell closed culture, reduce the chance of cell contamination and operator infection The gas exchange area is large, which is conducive to mass culture in limited culture
space.
The material is transparent and flexible, which is convenient for direct observation of the cell state EVA material under the microscope, in line with the Japanese Pharmacopoeia resin pharmaceutical container standard, there are two specifications of individual packaging and large packaging (a pack of 10 pieces) can be selected to culture human lymphocytes with culture flasks and culture bags, and the cell source is healthy adult PBMC
.
Day0~4OKT3 stimulation, Day4~15 expansion culture, respectively, using A, B, C, D four kinds of media
.
Cell concentration and proliferation multiple were determined on the day of culture on the day of culture, and the results were that the culture with a culture bag was on average more than
20% higher than the culture flask.