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Virus-Induced Gene Silencing in Nicotiana benthamiana and Other Plant Species

 Last Update: 2020-11-10
 Source: Internet
 Author: User

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high throughput

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Virus-induced gene silencing (VIGS) is an efficient tool for high throughput reverse genetic screens. VIGS engages the endogenous RNA-silencing machinery of the plant host, and can yield an 85–95% reduction of target transcripts. Gene silencing is rapid, target-specific, and does not require the creation of stable transformants. The technique has been used successfully in numerous Solanaceae species as well as in
Arabidopsis
, maize, and rice. Here we describe a protocol for conducting a VIGS screen in
Nicotiana benthamiana
using
Tobacco Rattle Virus
(TRV) based silencing vectors. This protocol can readily be adapted to many other model plant species.

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