Using improved MRS to identify glioma IDH mutation in the body

Ref: Branzoli F,et al.Neuro Oncol.
 2018 Jun 18;20 (7): 907-916doi: 10.1093/neuonc/nox214.)about 70-90% of grade II, Class III gliomas and glioblastomas (GBM) can have isiscite dehydrogenase (IDH) mutations, which are characterized by biological characteristics different from THOSE wild gliomasAt present, IDH has been recognized as the main marker for the prognosis of gliomahow to detect the state of the idh mutation in the glioma of the body brain by noninvasive methods has not been reportedFrancesca Branzoli, of the Institute of Neuroimaging at the Cerveau de la Moelle Institute in Paris, Francesca Branzoli, and others used magnetic resonance spectrum (MRS) imaging to detect metabolites 2-hydroxydoxic acid (2HG) to determine the status of the IDH mutation in patients with glioma, published in the June 2018 issue of NeuroOncolthis prospective study included 24 patients with low-grade gliomasMrS data was obtained on 3T MRI devices using 2 MRS imaging methods, improved MRS detection was performed through the Mescher-Garwood Resolution Spectrometry (MEGA-PRESS) sequence, and HG2 was detected using the espresso-optimized PRESS sequence of .6mLthen, automated immunohistochemical analysis is combined with Sanger sequencing to determine the idh mutation stateThe 2HG level of tissue samples was then measured by gas chromatography-mass spectrometry and compared with the MRS test resultsIt was found that the specificity and sensitivity of the improved MRS sequence in detecting 2HG reached 100%The 2HG level assessed by this technique is consistent with the 2HG level measured from tissue samples The performance of 2HG is lower to optimize the PRESS sequence detection the authors believe that improved MRS is a reliable means of non-invasive detection of IDH mutations, showing high sensitivity and specificity to 2HG detection Integrating improved MRS into clinical practice is beneficial to noninvasive diagnosis, prognosis and treatment plan for glioma (First Hospital of Fuzhou Serena compiled, Professor Wang Zhiqiu , of Huashan Hospital affiliated with Fudan University, editor-in-chief of "Outside The God Information" and Of Fudan University affiliated Huashan Hospital, Professor Chen Jicheng ) related links