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A chromatographic column is commonly referred to as a chromatographic column, which is a widely used method in chromatography
.
For mixed samples containing a variety of organic substances, recrystallization can be said to be an effective separation method in organic experiments when it cannot be purified by recrystallization
.
Based on the operating experience of using column chromatography for many years, this article summarizes several tips when using column chromatography: It is easy to install a long or short column at the beginning of column chromatography, and sometimes there will be a lot of silica remaining.
, waste of silica gel, which is mainly due to the lack of control over the amount of silica gel and other stationary phases used
.
The silica gel used for column chromatography is generally 100-200 mesh, and the mass of 100 ml of silica gel is about 47 grams.
If a column with a diameter of 2.
8 cm is installed, it can be installed with a height of 18 cm
.
In order to avoid wasting silica gel and solvent, it is necessary to investigate the columns of various specifications in the laboratory when learning to pack columns
.
The method is very simple: measure 100 ml of dry silica gel with a measuring cylinder, pour it directly into various columns of various specifications, tap it firmly, and measure the height of the silica gel in the column with a scale, so that you can know it well
.
Generally, when packing the column, the amount of silica gel used can be adjusted according to the height of the column required for the experiment, which can greatly save the use of silica gel and avoid unnecessary waste
.
When weighing silica gel, it is generally 30 to 70 times the sample amount.
If it is extremely difficult to distinguish, you can also use more than 100 times the amount of silica gel
.
With the development of science and technology, the technology of chromatography is also changing with each passing day, but the experimental technology of column chromatography is still relatively simple and practical.
To summarize and improve the experimental techniques of column chromatography
.
.
For mixed samples containing a variety of organic substances, recrystallization can be said to be an effective separation method in organic experiments when it cannot be purified by recrystallization
.
Based on the operating experience of using column chromatography for many years, this article summarizes several tips when using column chromatography: It is easy to install a long or short column at the beginning of column chromatography, and sometimes there will be a lot of silica remaining.
, waste of silica gel, which is mainly due to the lack of control over the amount of silica gel and other stationary phases used
.
The silica gel used for column chromatography is generally 100-200 mesh, and the mass of 100 ml of silica gel is about 47 grams.
If a column with a diameter of 2.
8 cm is installed, it can be installed with a height of 18 cm
.
In order to avoid wasting silica gel and solvent, it is necessary to investigate the columns of various specifications in the laboratory when learning to pack columns
.
The method is very simple: measure 100 ml of dry silica gel with a measuring cylinder, pour it directly into various columns of various specifications, tap it firmly, and measure the height of the silica gel in the column with a scale, so that you can know it well
.
Generally, when packing the column, the amount of silica gel used can be adjusted according to the height of the column required for the experiment, which can greatly save the use of silica gel and avoid unnecessary waste
.
When weighing silica gel, it is generally 30 to 70 times the sample amount.
If it is extremely difficult to distinguish, you can also use more than 100 times the amount of silica gel
.
With the development of science and technology, the technology of chromatography is also changing with each passing day, but the experimental technology of column chromatography is still relatively simple and practical.
To summarize and improve the experimental techniques of column chromatography
.
