Use and maintenance of the incubator

The incubator is the main equipment for culturing microorganisms, which can be used for the cultivation and reproduction of bacteria and cell.
The principle is to use artificial methods to create an artificial environment for the growth and reproduction of microorganisms, cells and bacteria in the incubator, such as controlling a certain temperature, humidity, gas,et.
There are four types of incubators currently in use: direct electric heating incubators, water-proof electric heating incubators, biochemical incubators and carbon dioxide incubator.
(1) Electric heating and water-proof incubator The shell of the electric-heating and water-proof incubator is usually made of asbestos board or iron sheet painting, and the inner layer of the water-proof incubator is a water storage interlayer made of copper ski.
The interlayer of the box is made of thermal insulation materials such as asbestos or glass wool to enhance the thermal insulation effec.
The top of the incubator is equipped with a thermometer, which is automatically controlled by a temperature controller to keep the temperature inside the box constan.
The water-proof incubator is heated by heating the water with an electric heating tub.
On the front side of the incubator, there are indicator lights and a temperature adjustment kno.
When the power is turned on, the red indicator light is on, and the knob is turned to the required scale according to the required temperatur.
When the temperature is reached, the red indicator light turns of.
Indicates that the required temperature has been reached in the box, after which the temperature in the box is automatically controlled by a reliable temperature controlle.
Use and maintenance: (1) The culture in the box should not be placed overcrowded to facilitate hot air convection, and the door should be closed whenever the items are put in or taken out to avoid temperature fluctuation.
(2) The electric incubator should put a water container in the box to maintain a certain humidit.
(3) The water-proof incubator should pay attention to adding water first and then turning on the electricit.
At the same time, the water level should be checked frequently and water should be added in tim.
(4) When the electric heating incubator is used, the wind top should be properly unscrewed to facilitate the adjustment of the temperature in the bo.
(2) Biochemical incubator This kind of incubator is equipped with electric heating wire heating and compressor cooling at the same tim.
Therefore, it can adapt to a large range and can be maintained at a constant temperature throughout the year, so it is gradually popularize.
The use and maintenance of this incubator is similar to that of an electric heating incubato.
Due to the installation of a compressor, the precautions for maintenance of the refrigerator should also be observed, such as maintaining a stable voltage, not tilting excessively, and cleaning the dust on the radiator in tim.
(3) Carbon dioxide incubato.
The carbon dioxide incubator is improved on the basis of ordinary culture, mainly by adding CO2 to meet the environment required for culturing microorganism.
Mainly used for tissue culture and the culture of some special microorganism.
Installation and debugging of CO2 incubato.
There is an operation panel on the front of the CO2 incubator, which is equipped with a power switch, a temperature regulator (manual and LCD panel), a CO2 injection switch, a CO2 adjustment knob, a humidity adjustment knob, a temperature display panel, a CO2 display panel and Humidity display plate, carbon dioxide sample hole (used to extract samples in the box to detect whether the CO2 in the box reaches the content displayed on the display plate) and alarm device (over-temperature alarm light.
(1) The incubator should be placed in a relatively stable position and away from heat sources to prevent temperature fluctuations and microbial contaminatio.
(2) Before turning on the power, a certain amount of distilled water should be added to the incubator according to the instruction manual (it is better to add a certain amount of disinfectant to the added water, see the instruction manual for details) to avoid burning the machin.
(3) When the water is added to a certain amount, the alarm light is on, that is, stop adding water, turn on the power switch, that is, start heating, and adjust the temperature controller to the required temperatur.

(4) When the temperature reaches the required temperature, the heating will be automatically stoppe.
When the temperature exceeds the required temperature, the over-temperature alarm light will be on and an alarm will soun.
(5) The CO2 used in the incubator can be liquid CO2 or ga.
No matter what kind of CO2 is used, the tube should not be too warped to ensure the smooth flow of the ga.
Generally, CO2 cylinders are used, and the pressure control gauge can be connecte.
(6) Zero adjustment of CO2 content After the temperature and humidity in the box are stable (usually three days), turn the CO2 adjustment knob to adjust the number of the display panel to 00, and repeat the adjustment if necessary after 5 minutes, until until the reading on the display is stabl.
Turn on the CO2 injection switch (the injection light is on), adjust the CO2 setting value to the required concentration, and after the concentration reaches the set value concentration (the injection light is off), keep it for at least 10 minute.
After that, the CO2 content in the box is automatically adjusted by the CO2 controlle.
(7) Adjustment of the humidity of the CO2 incubator Press the humidity adjustment knob first, and then turn the humidity adjustment knob to the desired cultivation humidity, and then the humidity is automatically adjusted by the humidity regulato.
Precautions for use (1) The incubator should be managed by a special perso.
Once any switches and adjustment knobs on the operation panel are fixed, do not twist them at will, so as not to affect the temperature, CO2, humidity fluctuations in the box, and reduce the temperature of the machin.
sensitivit.
 　　(2) The added water must be distilled water or non-ionized water to prevent minerals from accumulating in the water tank and causing corrosio.
The water must be changed every yea.
Always check that there is enough water in the tan.
 　　(3) The inside of the box should be regularly scrubbed and disinfected with disinfectant, and the shelf can be taken out for cleaning and disinfection to prevent other microbial contamination, resulting in the failure of the experiment
(4) Regularly check the over-temperature safety device to prevent over-temperatur.
The method is to press the monitoring alarm button, turn the fixing screw until the over-temperature alarm device rings, and then turn off the over-temperature safety ligh.
 　　(5) If carbon dioxide is not used for a long time, the CO2 switch should be turned off to prevent the CO2 regulator from malfunctionin.
 　　(6) The CO2 used must be pure, otherwise the sensitivity of the CO2 sensor will be reduced and the CO2 filter device will be pollute.
 　　(7) In an incubator without humidity control, in order to maintain the stability of CO2 in the box, a container containing water should be placed at the bottom of the bo.
 　　There is also a three-gas incubato.
This incubator is a new product that is further improved on the basis of the carbon dioxide incubato.
The principle is the same as that of other incubator.
The characteristic is that not only CO2, but also nitrogen and oxygen can be added, and the content of various gases is controlled and adjusted by compute.
It is mainly used for the reproduction and cultivation of some special microorganism.
It is not widely used in genera.
At present, it is only produced by a few foreign manufacturers, and the price is relatively expensiv.