Tianjin Institute of industrial biology has developed a new type of protease and its matching technology for gelatin preparation

Gelatin is the hydrolysate of collagen, which is widely used in more than 30 industries, such as medicine, food, textile, chemical industry, electronics, papermaking, printing, etc With the segmentation of gelatin Market, the quality requirements of gelatin have shown new characteristics of high standard, systematization and specialization Due to the long cycle, difficult to control the sanitary conditions, difficult to improve the equipment, and pollution to the environment, the traditional acid-base process is difficult to meet the system demand of the growing gelatin Market The western region of China is rich in raw materials and suitable for humidity, which is the main producing area of gelatin in China However, the acid and alkali emissions in the traditional gelatin acid-base preparation process cause serious pollution to water and air At the same time, the disadvantages of the traditional technology, such as large water consumption and high energy consumption, aggravate the shortage of water resources and electric energy in the western region Recently, the research team led by researcher song Xiao from Tianjin Institute of industrial biotechnology, Chinese Academy of Sciences, discovered a new protease that can be used for gelatin preparation by sequencing the macro genome of soil samples, and optimized the technology of gelatin preparation by protease based on the enzyme In this study, we used protease with independent intellectual property rights to prepare gelatin, and realized the biological substitution of chemical process The steps of multiple pickling, washing, liming and neutralization in the traditional preparation process of bone gelatin by alkali method were simplified into one-step reaction of acid enzyme treatment, and the process flow was shortened At the same time, the following extraction process was changed from the traditional multi-channel extraction process to two-channel extraction The yield of the first layer of gelatin was up to 12%, which was close to the total yield of the multi-channel extraction process of traditional acid-base method The total yield of gelatin prepared by enzyme method was also higher than that of the traditional method by 14-15%, which was up to 17% At the same time, compared with the gelatin prepared by the traditional acid-base method, the protein electrophoretic bands of the enzyme method gelatin are more concentrated and single, and the freezing force value of the obtained gelatin can reach more than 250 blog, and the viscosity value can reach 4.0Mpa · s, which is equivalent to the traditional method The total production cycle of gelatin was shortened from more than 60 days to 5 days, and the consumption of fresh water was reduced by 90% At the same time, the consumption of large amount of energy and chemical agents was reduced The green technology of gelatin preparation was realized The research has been supported by the National 863 program, and has applied for a number of national patents, two of which have been authorized (zl201320431570.7, zl201320431558.6).