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Three Methods for the Introduction of Foreign DNA into Agrobacterium

 Last Update: 2021-01-14
 Source: Internet
 Author: User

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The genetic manipulation of
Agrobacterium tumefaciens
is used to facilitate studies of bacterial gene functions or as a first step in introducing genetic material into transformable plant cells through the use of T-
DNA
binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into
Agrobacterium
using the triparental mating method. Here we present three detailed protocols for
Agrobacterium
strain construction using electroporation, the freeze/thaw method of transformation, and triparental mating.

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