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The cells were isolated and established
Cell Name: Human Monocyte Leukemia Cell
➊ Rehydration method: When the medium turns yellow, an appropriate amount (1~2mL) of fresh medium can be added, and after 1-2 times of replenishment, all the liquids can be changed by centrifugation, centrifugation at 1200 rpm (about 250g) for 3 minutes
➋ Semi-change method: Take the T25 bottle as an example, which contains 5 mL of medium
➊ Shake the flask, shake the cells well, divide them evenly into two bottles, and add an equal amount of medium
➋ Centrifugation method: Count after centrifugation, and seed into T25 bottles according to the density of 400,000-500,000 cells/mL, and the amount of medium in the bottle is 5 mL
➊ Since THP-1 is a suspension cell and is more susceptible to cryopreservation, it is recommended to increase the cryopreservation density, about 2 million to 3 million /mL, to improve the survival rate
Common problems and solutions for THP-1 culture
➊ Must I add β-mercaptoethanol β to my medium?
β-mercaptoethanol is a commonly used culture supplement that has antioxidant effects that reduce the effects
➋ What should I do if suspended cells are difficult to focus or estimate density when viewed under the microscope?
Suspension cells will flow with the liquid, not easy to focus, let stand for 5-10 minutes to sink the cells to the bottom, and then gently put on the microscope to observe, will help focus and estimate cell density
➌ What should I do if THP-1 cells clump during culture?
A small number of cells are clustered and grape bunches, which are normal phenomena, especially when the cell density is low, which is prone to occur
➍ What should I do if THP-1 cells adhere to the cells during culture?
A small amount of cell adherent is normal, and the cell suspension can be transferred
