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Thin layer analysis and paper layering.

 Last Update: 2020-10-27
 Source: Internet
 Author: User

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< ( 70,135,221); To < the basic principles of > ofand the use of thin layering and paper

2. Learn and master the operation methods of thin layer analysis and paper layering

II, experimental principle: the basic principle of

chromatography is to use the various components in the mixture in a substance adsorption or dissolution of different properties. Or other affinity performance differences, so that the solution of the mixture flows through the substance, repeated adsorption or distribution, etc. , so as to separate the components.

use: separation, purification, identificationfororganic objects.

" the maximum concentration center of the solute to the center of the original point

Solvent Frontier to The Center of Origin

acetylene, sultan III, acetaminophen and sultan three mixtures, 9:1 cytohexane: ethyl acetate, tianmen dongeine methionine,tritone ethanol solution; thin sheet, capillary tube, expanded cylinder, 10:50:2 water-ethanol-ice acid.

4, operation steps:

(i) thin layer analysis: the separation of anitrobenzene and Sudan III

< Preparation of the >

sheet: Mix 3g silicone G with 5% sodium methyl cellulose (CMC) aqueous solution to paste and dump it on a clean glass sheet to make a uniform, flat sheet. After placing 0.5h at room temperature, remove the spare < 1100C, 0.5h > oventhe oven.

point sample: at a distance of 1.5 cm at one end of the thin sheet, gently draw a line starting line with a pencil, and use capillary tubes to point on anitrobenzene, mix sample, Sudan III.

unfolded: with 9:1 cyclane-ethyl acetate as an expander, after the sample point driding, put in the expander bottle to expand (note: the expander is not easy to over-expand, so that the sample is immersed in 0.5cm is appropriate) When the expander reaches 1cm at the end of the sheet, remove the sheet and use a pencil to draw the solvent. Observe the separation to calculate and compare the RF values of both.

(ii) Paper analysis: Tianmen winter > amino acids and methionine separation

in a specific expander distribution coefficient is different, using standard samples and samples on the same sheet of dialysis paper for layering. Compare their Rf values for the purpose of isolating and identifying amino acids.

< the "list-paddingleft-2" >

requires a smooth and uniform thin layer with no cracks.

do not puncture the sheet when

solvent is not easily placed in the expander bottle

do not mix the

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