The Zeng Hui/Zhao Heart collaboration study revealed that the cell receptor pool of T-damaged patients with HIV/AIDS after long-term ART treatment could not fully recover.

Antiretroviral therapy (Art) can effectively improve the number of CD4 + T cells and immune function of HIV / AIDS patients, so as to protect patients from AIDS related diseases.however, it has been found that the incidence of AIDS related non related diseases in HIV / AIDS patients after long-term art treatment is much higher than that in normal people.it is suggested that the immune function of CD4 + T cells or the diversity of TCR still exist after art treatment.at present, a large number of studies have focused on the study of the immunosuppressive status of CD4 + T cells in HIV / AIDS patients.there are few studies on the changes of TCR receptor Library of CD4 + T cells in patients with HIV infection and treatment, especially the evaluation of TCR diversity of CD4 + T cells after long-term art treatment.in the previous studies on TCR receptor Library of T cells from HIV / AIDS patients with ngs, the main research strategy was to analyze the characteristics of HIV antigen-specific TCR CDR3, and there was no description of the overall change of TCR diversity. Recently, he published a research paper on HIV / AIDS in Beijing University V-J pairing instead of CDR3 as a macro indicator, describes the change of CD4 + T cell receptor diversity from damage to recovery after HIV infection to art treatment, and reveals that although long-term art treatment can increase the number of CD4 + T cells in HIV / AIDS patients, its damaged TCR receptor library cannot be fully recovered (Figure below).in the course of the study, the researchers noticed that TCR V-J pairing expression in healthy people had a preference.through data analysis, it is found that TCR V-J pairing preference is highly conservative in healthy individuals and among individuals.the similar V-J pairing preference among different individuals provides a scale to evaluate the overall change of TCR diversity after T cell system is hit.the researchers have established a set of evaluation system including five categories (coverage, top 10% cumulative percentage, diversity, intra individual similarity and inter individual similarity) parameters to evaluate the overall change of TCR receptor library diversity of CD4 + T cells after HIV infection and long-term art treatment ().as expected, most of the V-J pairing parameters of HIV infected patients who did not receive art changed significantly; moreover, after more than five years of long-term art, with the recovery of CD4 + T cell number, most of the V-J pairing preference parameters of CD4 + T cells in patients can also return to the level of healthy people.however, it is surprising that the long-term art treatment still makes the similarity of TCR V-J pairing of CD4 + T cells between patients and healthy people still has significant difference.it is suggested that the types of TCR V-J pairs in patients with restoration of TCR diversity are different.in these long-term art treatments, most (240) of the V-J pairs with different expression were found in the α chain of Na ï vecd4 + T cells reconstructed after HIV attack.and art treatment itself can also lead to changes in the expression of some TCR V-J pairs.among these V-J pairing α chains with different expression levels, those affected by HIV but not recovered by art therapy were mainly concentrated in trav38-2 / dv8, trav8-2, trav8-4, trav38-2 and trav8-4 The expression of trav38-1 and trav12-1 was highly correlated with each other (Fig. a, b). These phenomena did not appear in the changes of V-J pairing induced by art treatment (Fig. C, d).this study has important implications for assessing whether the deficiency of TCR V-J pairing after long-term art treatment is associated with the high risk of AIDS related cancer (nadc) or some infections.Art drugs may affect the rearrangement of TCR V and j fragments, which also has certain research value for clinical art treatment plan and timing.Beijing Ditan Hospital Affiliated to Capital Medical University is the first author of this paper, and Professor Zeng Hui, Professor Zhao Hongxin and Professor Chen Chen Chen are the corresponding authors.Article Information: [click the link below or "read the original text" to get the full text] Li, g., Li, J., Zhang, H., Zhang, Y., Liu, D., Hao, Y., Han, J., Du, J., Zhu, L., Zeng, Y., et al. (2020). Partial recovery of disturbed V-J pairing profilesof T-cell receptor in people living with HIV receiving long-term antiretroviral therapy. Sci China Life Sci 63,1718-2