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Recently, the "primers, methods and kits for the identification of bluefin tuna based on SNP markers" researched and invented by Yao Lin of the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences have been authorized by the national invention patent, patent number: ZL 2020114005862
.
.
The present invention is based on the end of 2b-RAD simplified genome sequencing, mining species-specific SNP sites of bluefin tuna, and providing a complete set of primers for the identification of bluefin tuna based on species-specific SNP molecular markers of bluefin tuna, which includes a pair of PCR amplification primers and a circulating probe
designed based on the SNP molecular marker.
The SNP molecular marker includes a G base located at position 808 in the SEQ sequence or a base located at position 220 in the SEQ sequence
.
The present invention also provides a method and kit
for identifying bluefin tuna based on a set of primers.
The set of primers, methods and kits of the present invention can quickly identify or identify bluefin tuna, which has the characteristics of
simple operation, low sampling amount, short detection cycle, good method specificity and high sensitivity.
designed based on the SNP molecular marker.
The SNP molecular marker includes a G base located at position 808 in the SEQ sequence or a base located at position 220 in the SEQ sequence
.
The present invention also provides a method and kit
for identifying bluefin tuna based on a set of primers.
The set of primers, methods and kits of the present invention can quickly identify or identify bluefin tuna, which has the characteristics of
simple operation, low sampling amount, short detection cycle, good method specificity and high sensitivity.
