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Fully automated, multifunctional, ultra-sensitive fluorescence Western revolutionizes Western Blot technology
ProteinSimple officially released the Stellar Ultra-sensitive Fluorescence Detection Kit in the world, which is carried on the fully automatic multifunctional ultra-sensitive fluorescence Western protein analysis platform Jess (Digital Western Blot), providing automated highly sensitive fluorescent immunoprotein detection technology solutions
.
This technology scheme is especially suitable for cell signaling pathway and pharmacological efficacy research, simultaneous detection of phosphorylated proteins/total proteins with similar molecular weights, realizing the ability to run multiplex protein expression analysis in one run, and achieving accurate quantification
of low-abundance proteins in complex samples with high sensitivity.
The detection of phosphorylated proteins by traditional chemiluminescence and fluorescence Western Blot methods is a time-consuming and laborious task
.
In order to solve the traditional technical challenges and overcome the well-known limitations of traditional western blotting, ProteinSimple has developed a fully automated multifunctional protein expression quantitative analysis technology platform, coupled with ultra-sensitive fluorescence detection technology, which can more easily and efficiently complete phosphorylated protein detection
in cell signaling pathway research.
Stellar ushers in a new era of automated fluorescence Western
Figure 1 Stellar fluorescence detection sensitivity is achieved
through multiple signal amplification steps in which DNA is connected to the detection antibody.
The fully automated, versatile ultrasensitive fluorescence detection kit Stellar continuously amplifies immune response signals using patented non-enzymatic oligonucleotide amplification technology and provides ultra-high fluorescence sensitivity at detection levels below 1 pg with Jess's NIR/IR fluorescence detection, as well as excellent reproducibility and 4-log dynamic range
.
With this leap in sensitivity, Stellar fluorescence detection is comparable to widely recognized chemiluminescence detection sensitivity and replaces fluorescence detection that requires traditional western blot imaging techniques at 50pg detection
levels.
Advantages of fully automatic multifunctional ultra-sensitive fluorescence Western technology
◆Multichannel immunodetection using standard western blot antibodies in chemiluminescent, infrared (IR), and near-infrared (NIR) fluorescence channels for channel-based and size-based multiplex protein characterization;
◆ Fully automatic, ultra-sensitive fluorescence Western: can obtain the most data from trace samples (as low as 3 μL sample requirements), and quickly obtain results in as short as 3 hours, with more accurate quantification, higher repeatability, time-saving and labor-saving;
◆ Equipped with RePlex™ technology to perform two consecutive immunoassays in the same capillary, detect more target proteins or pass the total protein content normalization analysis, which is more reliable than using reference protein;
◆ To solve the problem of misuse or counterfeiting of traditional Western blot pictures, Digital Western blot system software meets FDA CFR Part 11 compliance standards, the whole process of tracking records, the original records cannot be tampered with
.
◆ Scientists widely recognize that the fully automated Simple Western technology platform, or Digital Western Blot, has been used in nearly 2,000 high-impact articles and is a proven technology with authority and reliability
.
Figure 2 AKT total/phosphorylated protein
for multiplex detection by Stellar NIR and IR.
Lysate (0.
2 mg/mL) from Jurkat cells (treated with calyculin A) and probed
using mouse anti-total AKT and rabbit anti-phosphate AKT primary antibodies as well as Stellar Mouse IR (green band) and Stellar Rabbit NIR (red band) detection modules 。 The Stellar NIR/IR channel is capable of multiplexing total AKT and phosphorylated AKT in the same lane with excellent reproducibility (24 lanes: pAKTs <10% CVs and Total AKTs <5% CVs).
About us
ProteinSimple is the industry-leading protein analysis brand
owned by NASDAQ-listed Bio-Techne Group (NASDAQ: TECH).
。 We are committed to developing and producing more accurate, faster and more sensitive innovative protein analysis tools, including protein charge characterization, protein purity analysis, quantitative detection of protein post-translational modifications, protein immunoassays such as Western and ELISA quantitative detection of protein expression, etc.
, to help scientists in the fields of vaccine development, biopharmaceuticals, cell therapy, gene therapy, biomedical and life sciences solve protein analysis problems and deeply analyze the relationship between proteins and diseases
。
Contact us
Address: Room 1901, Building 3, Raffles City Plaza, No.
1193 Changning Road, Shanghai
Tel: 021-60276091
Hotline: 4000-863-973
E-mail: PS-Marketing.
CN@bio-techne.
com
Website: www.
bio-techne.
com
