The technology of freeze-drying beads and the solution of automation system

Biological reagent preservation is an important problem in the medical industry Biological reagents are bioactive substances, which are particularly sensitive to temperature The chemical properties are relatively active and easy to react with each other Because of the above characteristics, the storage time of biological reagent is generally short and needs to be frozen, otherwise it will deteriorate quickly In addition, many biological reagents (such as nucleic acid detection reagents) are required to be stored at room temperature, which requires that biological reagents cannot be stored in liquid form and need to be dehydrated The methods of reagent dehydration are as follows: 1 Dispensing and drying; 2 Liquid nitrogen freeze-drying technology The dispensing drying adopts the technology of heating and drying to evaporate the water in the reagent through the method of vacuum and high temperature heating But this simple dehydration method has great disadvantages High temperature may affect enzymes and proteins, and they are relatively insoluble The lyophilized bead technology uses the technology of freeze-drying to dehydrate the reagent The lyophilized bead technology can maintain the activity of enzyme / protein to a large extent, and the lyophilized bead has a loose network structure and rapid re dissolution The technology of lyophilized beads can transform unstable chemical reagents into high quality, stable and quantitative lyophilized beads The technology of liquid nitrogen freeze-drying beads is to drop the reagent liquid in the device with liquid nitrogen, so that it can be solidified into pellets in a very short time under low temperature environment Liquid nitrogen, liquid nitrogen, is inert (not reacting with other substances), colorless, odorless, noncorrosive, nonflammable, extremely low temperature liquid Its excellent characteristics and reasonable price determine that liquid nitrogen is an ideal low-temperature freezing medium After the liquid drops into liquid nitrogen to form a ball, the ordinary freeze-drying machine with controllable cost can be used for freeze-drying In this process, the pressure needs to be controlled At present, the main technical difficulties of liquid nitrogen freeze-drying bead technology are: 1 Micro point solution: liquid nitrogen freeze-drying beads generally need micro point solution, and the common point solution quantity is from 1 μ L (1 mg) to 10 μ L People are familiar with the use of ordinary pumps, it is difficult to maintain a stable point liquid in such a small range and ensure consistency 2 Precise spot solution: the lyophilized beads have higher requirements on the precision and shape of spot solution When the laboratory is operating, it is difficult to control the accuracy and consistency due to the problems of manual consistency and simple equipment If the ordinary pump is used, the controllability and accuracy of the pump are not good when the micro point liquid is used, which is very easy to cause the shape and size of the small ball are different, the shape of the ball is not good, or the small ball explodes into multiple microspheres when it falls on the surface of liquid nitrogen When the amount of liquid is small, it can't form a ball with the same shape, and when the amount is large, the lyophilized bead is difficult to re dissolve If the shape and size of the lyophilized beads are different, the subsequent detection process and results will be affected In order to solve the above problems, the technical team of Guangzhou feisheng precision equipment Co., Ltd has been focusing on the research and development of micro (micro level and nano level) fluid control system since 2010, Based on the technology of micro fluid pump, ds228 desktop system and ds328 floor standing system which are suitable for laboratory / small-scale production and large-scale production of liquid nitrogen freeze-dried beads and beads have been successively launched The volume has reached the level of 1 micro liter, which is Taiwan, Well known bioengineering companies in Shenzhen, Chengdu, Tianjin, Ningbo, Zhuhai and other regions have provided system solutions for semi-automatic and automatic production of liquid nitrogen freeze-dried beads, becoming professional system suppliers in this application field Ds228 desktop liquid nitrogen lyophilized bead production system ds328 floor standing liquid nitrogen lyophilized bead production system ds228 and ds328 liquid nitrogen lyophilized bead system adopt the precise micro ceramic pump technology with the point liquid volume as low as 1 μ L, and the components contacting with the liquid medicine are sapphire ceramic, Teflon and 316 Stainless steel materials, these materials will not react with the liquid medicine, thus ensuring the activity, stability and non pollution of the liquid medicine Ds228 and ds328 The volume and speed of the system can be adjusted quickly In addition, the company can customize all kinds of special needles, which can achieve the rate of liquid dispensing The liquid dispensing does not hang drops, and does not explode when it is put into liquid nitrogen The lyophilized beads have good circular effect, uniform shape and volume, and the same size, which effectively solves the problem that the lyophilized beads are easy to hang drops or explode, with inaccurate accuracy and spherical effect commonly faced by biochemical customers The problem of poor fruit and consistency Guangzhou feisheng company has a variety of specifications of micro ceramic pump to choose from, as low as 1 μ l to more than dozens of μ l point liquid volume range can achieve high-precision point liquid, point liquid precision as high as 0.5%, can meet the customer's various point liquid requirements The system of lyophilized beads with diameter ≥ 1mm ds228 and ds328 can realize automatic production The parameters of automatic production can be simply set through two screens The display 1 is the micro pump controller, which is mainly used to control various liquid point parameters of the micro pump Display 2 is the system operation panel, which is mainly used to control the overall parameters of the whole liquid nitrogen lyophilized bead system Ds228 and ds328 systems can be equipped with single channel or multi-channel micropump point liquid system Single channel micropump system is suitable for laboratory or small batch production, and multi-channel micropump system is suitable for batch production Ds228 and ds328 systems are designed with unique mechanism, which is convenient for quick collection and storage of lyophilized beads The system is equipped with integrated liquid nitrogen tank and optional safety liquid nitrogen filling device Ds228 and ds328 systems have the advantages of micro (1 microliter), high precision (0.5%), rate (100-200ppm), high consistency of lyophilized beads, and automatic continuous production technology They are the system of lyophilized beads of biochemical company Guangzhou feisheng precision equipment Co., Ltd is a high-tech enterprise specializing in precise fluid control system and solutions Its professional technical team has served sannuo biology, Aikang biology, Tianjin micro nano core, Meikang biology and other bioengineering, pharmaceutical, medical equipment fields, For China and Asia customers to provide point liquid, marking, spraying, filling and other micro fluid control system solutions