The team of Academician Fu Tingdong reveals the new...

Nanhu News Network (Correspondent Wang Gang) On July 14th, Plant Biotechnology Journal published online the research group on the utilization of rape heterogeneity, led by Academician Fu Tingdong of Huazhong Agricultural University, entitled " Increased seed number per silique in Brassica juncea by deleting cis-regulatory region affecting BjCLV1 expression in carpel margin meristem "research paper

According to researchers, increasing yield is the eternal goal of rape genetic improvement

Researchers found that the mature siliques of common rape are composed of two siliques.

Comparing the phenotypic differences between the two rapeseeds during the whole growth period, it was found that only in the fifth stage of flower development (that is, the carpel primordium occupies the flower meristem), the fusion site of the two carpel primordia contained a meristem The characteristic tissue is the carpel margin meristem (CMM).

The other gene mc2 of the two recessive nuclear genes that control the traits of three-compartmental siliques was cloned into the other gene mc2, which is an Arabidopsis CLV1 homologous gene BjA7.

Transgene complementation verification must contain the full-length promoter of the BjA7.

Collinearity analysis of the promoter regions of CLV1 homologous genes in cruciferous species showed that the 914-bp deletion sequence of the mc2 promoter was very conserved in the promoter regions of these homologous genes

The main innovation of this study is to explain the reason why the phenotype of J163-4 is different from other multi-compartmental rapeseeds through phenotypic and molecular biological evidence.

Wang Gang, a doctoral student in the School of Plant Science and Technology of Huazhong Agricultural University, is the first author of the paper, and Professor Shen Jinxiong is the corresponding author of the paper

Original link: https://onlinelibrary.

Reviewer: Shen Jinxiong