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The Tao Wei laboratory of the School of Life Sciences cooperates with collaborators to reveal information about the in vivo activity of artificial chromosomes

 Last Update: 2022-01-09
 Source: Internet
 Author: User

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Information storage artificial chromosomes (data-carrying chromosome, dChr) can be used for data storage in a stable and inexpensive way to replicate

Studies have proved that dChr forms chromatin in yeast cells and forms Rabl structure together with yeast endogenous chromatin; dChr information sequence is coupled with multiple DNA elements related to high gene expression, such as 54 TATA boxes and 36 promoters DNA and multiple transcription factor binding sites, accompanied by transcriptionally active histone H3K4 trimethylation; 44 obvious H3K4 trimethylation peaks (peaks) detected on dChr indicate that dChr in yeast cells There may be high-efficiency transcription; further, 488 transcripts were detected by full-length transcriptome sequencing (Iso-seq).

Information on the genetic elements of artificial chromosomes

Tianjin University Zhou Jianting, Peking University graduate students Zhang Chao and Wei Ran are the co-first authors of this article, Tao Wei and Yuan Yingjin are the corresponding authors, Peking University researcher Li Cheng has given great help to the research, and the researcher Zhang Lihua of Dalian Institute of Chemistry, Chinese Academy of Sciences The group participated in the work of protein/peptide mass spectrometry prediction

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