The study found that the new endocrine peptide stimulates the plant's PTI immune response and disease mold resistance

Recently, Journal of Integrative Plant Biology published online a research article by a team of single-satellite professors at the Agricultural College entitled "The novel peptide NbPPI1 identified from Nicotiana benthamiana triggers immune responses and enhances resistance againstst Phytophthora pathogens".
, Ph.D. student at the School of Plant Protection, is the first author of the thesis, and Professor Single Satellite is the author of the paper newsletter.
plant immunity (PAMP-Triggered Immunity, PTI) activated by pathogen Associated Molecular Pattern (PAMP) based on the out-of-cell identification conservative molecular pattern of trans-membrane receptors (Pattern Recognition Receptor, PRR) is an important underlying resistance mechanism for plants.
in addition to identifying conservative molecular patterns of germs,
transfilmsomes are also able to identify their own risk signals of damage or bacterial contamination, triggering a plant immune response called damage-related molecular patterns (Damage-AssociatedMolecular Pattern, DAMP).
the transmeral receptors found in these areas are membrane receptor kinases (RKs) or receptor-like proteins (Receptor-Like Proteins, RLPs), indicating that plants are conservative in their immune response to pathogenic microbial infestion.
to identify the molecular patterns associated with damage associated with bacterial infection in plants, and to understand their biological function, it is expected to explore the improvement of broad-spectrum disease resistance of plants based on immune identification.
in the previous study, the single satellite professor team obtained NtPROPPI, a secretion peptide derived from tobacco that stimulates autoimmune response, through functional identification.
In this study, through sequence comparison and evolutionary analysis, the two entogenous genes NbPROPPI1 and NbPROPPI2 of NtPROPPI were identified in this tobacco, and the expression of their encoded genes was further confirmed by real-time quantitative RT-PCR method to respond to the induction of plague mold infection and salient acid treatment; The immunoactivation functions of NbPROPPI1 and NbPROPPI2 were confirmed, which, as molecular patterns associated with damage associated with plague mold infection, activated plant PTI immune response, including promoting thedeposition and reactive oxygen bursts caused by flog22, activating MAPK phosphate, expression of PTI pathway-related genes WRKY33 and FRK, etc., which can significantly enhance tobacco resistance to mold infestion.
For conservative segments, NbPPI1 and NbPPI2 are chemically synthesized on the carbon end of NbPROPPI1 and NbPROPPI2, respectively, the peptide segments NbPPI1 and NbPPI2 are chemically synthesized, and the external source applies the same enhancement of plant resistance to parasitic and pathogenic molds.
further analysis showed that the immune function of NbPPI1 depended on the immuno-co-subject BAK1, and the peptide cut-off test showed that the eight amino acid residues specific to the carbon end of NbPROPPI were the key areas for activating the immune response.
research found that the peptide NbPPI derived from tobacco, as a molecular model associated with damage associated with the infection of plague mold, can stimulate the resistance of plants to epidemic mold, and lay the foundation for improving the resistance of crops to epidemic mold through biotechnology breeding.
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