The steps for the resuscation of microbial strains.

Biotechnology Channel News: Bacteria active is to preserve the state of the species into the appropriate medium culture, step-by-step expansion of culture is to obtain pure and strong culture, that is, to obtain a vigorous, adequate number of inoculation culture.
species fermentation generally needs 2-3 generations of resuscing process, because the conditions at the time of preservation and culture conditions are often not the same, so to be active, so that the bacteria gradually adapt to the culture environment to understand the situation of species resuscation needs to understand the way and method of species preservation.
current commonly used in the international and domestic species preservation methods include: regular transplantation method, liquid paraffin method, sand pipe method, vacuum freeze-drying method, 80 degrees C refrigerator freezing method, liquid nitrogen ultra-low temperature freezing method.
different ways of resuscitation for different preservation methods: 1, the resuscitation of bacteria by regular transplantation method is relatively simple, direct transfer can be; 2, liquid paraffin preservation of bacteria in the recovery, pick a small number of bacteria transferred to the appropriate fresh media, growth and reproduction, and then re-transfer once;
or take sand grains in a suitable liquid medium, multiply culture and then transfer the slope; 4, vacuum freeze-drying method to preserve the bacteria in the recovery of the first 70% alcohol cotton wipe the upper part of the ampoule, the top of the ampoule tube hot, with sterile cotton swabs cold Water, wipe a circle at the top, cracks appear on the top, tap with a knife or tweezer neck, knock down the top of the cracked ampoule tube, dissolve the bacteria with sterile water or culture solution, use a sterile straw into a fresh culture base, for temperature culture; 5, -80 degrees C refrigerator freezing method to preserve the recovery of bacteria, remove ampoule tube or plastic freezer tube from the refrigerator, should immediately place 38 degrees C-40 degrees C water bath to quickly recover and shake appropriately quickly.
until all the internal ice dissolves, it takes about 50-100 seconds.
Open the ampoule tube or plastic freeze tube, move the contents to a suitable medium for culture 6, liquid nitrogen ultra-low temperature freezing method preservation bacteria recovery and -80 degrees C refrigerator freezing method preservation bacteria recovery is similar, from the liquid nitrogen tank to remove ampoule tubes or plastic freezers, should be immediately placed in the 38 degrees C-40 degrees C water bath rapid recovery and appropriate shake.
until all the internal ice dissolves, it usually takes about 50 to 100 seconds.
the ampoule tube or plastic freezer tube and move the contents to a suitable culture base for culture.
the following steps are needed to summarize the activity of the bacteria: the first configuration of the bacteria suitable for growth of the culture base; The second restores the species from the storage state to the room temperature state, such as thawing the frozen species, the third will be through the operation of the protected species inoculated into the medium culture, this step is called the bacteria resuscation, the fourth step is to select the culture medium thriving bacteria, select some of the bacteria inoculated into the new medium culture, repeat this step 2-3 times, so as to get a good growth of the bacteria.
these four steps to reach the purpose of resuscing the species from the state of preservation.
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