The source of citrate in bone microstructures has been identified for the first time.

Recently, the Scientific Institute of Advanced Technology of the Chinese Academy of Sciences, the Institute of Biomedicine and Technology, the Institute of Management Sensitivity Research Group, the Chinese Academy of Sciences Hefei Institute of Material Science, the Strong Magnetic Field Science Center Wang Junfeng Research Group, in cooperation with the metabolic regulation interstitial hyperstature stem cell bone mechanism research progress.
-in-the-statistema stem cells (MSC) are stem cells with the potential of self-renewal and multi-directional differentiation, and are widely used in cell therapy for a variety of diseases, especially in bone repair and regeneration.
citrate is an important component of bone microstructure phosphorite nanocrystals, which affects bone strength, hardness and toughness.
but the mechanism of citrate production and deposition in the bone is not yet known in the current study.
MSC differentiation is closely related to cell glycel metabolism, and citric acid is an important intermediate product of the cell mitochondrial triamal acid cycle.
in this study, the researchers used liquid and solid nuclear magnetic resonance technology, through 13C isotope marker tracking and targeting metabolomics methods, found that the citrate in the phosphoric acid from the MSC bone differentiation later triamcinic acid cycle process glucose oxidation metabolism intermediate products, and revealed that MSC absorption using zinc ions to regulate the bone differentiation of Runx2/Osterix/ZIP1 molecular signal mechanism, this positive feedback pathway further promotes the formation of citrate and sedimentation.
the first study to discover the source of citrate in bone microstructure, revealthe the new mechanism of metabolic regulation and metabolism intermediates involved in bone formation, and provide an important theoretical basis for zinc ions as active ingredients to prepare new materials for bone repair.
related research in The Journal of Advanced Science, based on the research of Runx2/Osterix and Zinc Uptake Synergize to Orchestrate Osteogenic and Citrate Containing Bone Apatite Formation (Runx2/Osterix and zinc ion co-regulation interstitial stem cell differentiation and citric acid metabolism to form ostitatric ashstone).
the research was supported by the NSFC, the Ministry of Science and Technology, the Youth Innovation Promotion Association of the Chinese Academy of Sciences, the Shenzhen Science and Technology Program and the Peacock Team, as well as the MRI spectrometer system of the stable-state strong magnetic field experimental device.
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