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Isolation from solid tissues is the main source of animal cells.
Tissue obtained directly from an organism is closer to the living state in the organism, and its biological properties have not changed much, so it is very popular in many fields such as drug screening, cell transplantation, organoid culture, and tumor research
Cells grown in a monolayer have protein contact with each other and with the culture dish.
Figure 2.
- Small-scale cell harvesting can be done using pipetting or cell scrapers
- For the formation of protein tight junctions, trypsin can be used
- Cells that can express cadherin such as epithelial cells need to add chelating agents such as EDTA on the basis of enzymatic digestion
To maintain cell viability, digestion reaction conditions should match the intensity of cell contact
If the strength of the reaction is matched to the adhesion properties of the cell line, but the cells are still difficult to remove, trypsin-inhibiting elements, such as serum, may be present in the growth medium, which can be achieved by simply rinsing with PBS before adding the dissociation reagent cells once or twice to overcome
There is no doubt that trypsin, as one of the indispensable raw materials for primary cell acquisition and cell passage digestion, is also an important production raw material in biopharmaceutical processes
Zhudian Trypelectase Recombinant Trypsin and Tryplus Digestion Solution are trypsin products that focus on cell digestion and are specially designed for process and production
Trypelectase non-animal-derived recombinant trypsin dry powder product is convenient for large-scale production and convenient for storage and transportation
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