The role of hydrogen peroxide enzyme and peroxide enzyme.

, experimental purpose
1. To understand the role of hydrogen peroxide enzyme, master the commonly used methods to determine hydrogen peroxide enzyme.
2. To understand the role of peroxidase, master the commonly used methods of determining peroxidase.
, the experimental principle
oxide enzyme is a common presence in plants, high activity of an enzyme. It is closely related to respiratory action, photodepending and oxidation of growth hormone, etc. , in the process of plant growth and development, its activity is constantly changing, so measuring this enzyme can reflect the changes in plant metabolism in a certain period of time under the peroxidase catalysis, H2O2 will be more invasive wood phenol oxidized into tea brown products. This product has maximum light absorption at 470nm, so the activity of peroxidase (the more invasive wood phenol method) can be determined by measuring the change in absorbance at 470nm.
hydrogen peroxide enzyme is found in all
tissues
plants, and its activity is related to the metabolic strength and resistance of plants to cold and disease. Hydrogen peroxide enzyme belongs to hemoglobinase, contains iron, it can catalytic hydrogen peroxide decomposition into water and molecular oxygen, in the process of transmitting electrons, hydrogen peroxide is both an oxidant and a reducing agent.
, the enzyme's vitality can be determined based on H2O2 consumption or 02 production.
A certain amount of hydrogen peroxide solution (excessive reaction) is added to the reaction system, and after an enzymatic reaction, the excess hydrogen peroxide can be determined by titring the excess hydrogen peroxide with the standard potassium permanganate solution (under acidic conditions).
III, instruments, raw materials and
reagentsi) to determine hydrogen peroxide enzyme
instrument 722 type

,
centrifuge
, research,
capacity bottle
,
test tube
,
strawraw materials
potato block.
reagents
. 1. 0.05mol/LpH 5.5 phosphoric acid buffer;
2. 0.05mol/L healing wood phenol solution;
3. 2% H2O2;
4. 20% trechloroacetic acid.
(ii) Determination of peroxidase activity
instrument
research, triangular bottle, acid titration tube,
ringing temperature
water bath pot, capacity bottle
raw materials
wheat leaves
reagents
1. 10%H2SO4
2. 0.2mol/LpH7.8 phosphoric acid buffer
3. 0.1mol/L potassium permanganate standard liquid: KMnO43.1605g, 1000mL with freshly boiled cooling distilled water, then 0.1mol/L herbal solution calibration.
4. 0.1mol/LH2O2: Commercially available 30%H202 is approximately equal to 17.6mol/L, take 30%H2O2 solution 5.68mL, dilute to l000mL, and label with standard 0.1mol/LKMnO4 solution (under acidic conditions).
5. 0.1mol/L grass acid: said to take the excellent grade pure H2C2O4.2K2O12.607g dissolved with distilled water, fixed capacity to 1000mL.
.