The regulatory effect of AMPK on apoptosis of bronchial epithelial cells induced by cigarette smoke extract
-
Last Update: 2014-12-26
-
Source: Internet
-
Author: User
Search more information of high quality chemicals, good prices and reliable suppliers, visit
www.echemi.com
Recently, researchers from the respiratory department of Xiangya Hospital of Central South University published a paper aiming at endoplasmic reticulum stress is one of the important mechanisms leading to apoptosis in the course of chronic obstructive pulmonary disease This study explored the regulatory role of AMPK in the apoptosis of bronchial epithelial cells induced by cigarette smoke extract The results showed that CSE could induce apoptosis of NHBE cells in a time-dependent and concentration dependent manner, and the expression of AMPK might have a protective effect on cell resistance to CSE induced ER stress This article was published in the 29th issue of Chinese Journal of modern medicine in 2014 The experiment was divided into four groups: blank control group, AMPK specific inhibitor (compound C) group, CSE group and CSE + compound C group Human bronchial epithelial cells (NHBE) were cultured in vitro and treated with CSE and compound C respectively The expression levels of CHOP, Caspase 4 and p-AMPK protein were detected by Western blot The morphological changes were observed by Hoechst33342 staining and the apoptosis rate was detected by flow cytometry Compared with the control group, the expression of p-ampk / AMPK protein in CSE intervention group decreased significantly (P0.05).
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to
service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.
