The Production Process of 4-(4-Aminobenzyl)piperazine-1-carboxylic acid tert-butyl ester

The production process of 4-(4-Aminobenzyl)piperazine-1-carboxylic acid tert-butyl ester, also known as GV-976, involves several steps, which are outlined below:

Step 1: Synthesis of 4-Aminobenzyl Piperazine
The first step in the production process of GV-976 involves the synthesis of 4-aminobenzyl piperazine.
This compound is synthesized by treating a solution of benzaldehyde and piperazine in the presence of an acid catalyst, such as hydrochloric acid.
The reaction is then condensed and the resulting product is extracted with a solvent, such as ether or ethyl acetate.
The resulting 4-aminobenzyl piperazine is then dried and purified to obtain a pure sample for the next step.

Step 2: Synthesis of 4-(4-Aminobenzyl)piperazine-1-carboxylic acid
The next step in the production process of GV-976 involves the synthesis of 4-(4-aminobenzyl)piperazine-1-carboxylic acid.
This compound is synthesized by treating 4-aminobenzyl piperazine with an appropriate carboxylic acid, such as benzoic acid or acetic acid, in the presence of a base, such as sodium carbonate or potassium hydroxide.
The reaction is then allowed to proceed for a suitable period of time and is then quenched with a base, such as sodium bicarbonate or magnesium carbonate.
The resulting product is then extracted with a solvent, such as ethyl acetate, and the organic layer is dried and purified to obtain a pure sample for the next step.

Step 3: Estimation of GV-976
The final step in the production process of GV-976 involves the estimation of the compound.
This can be done by using a suitable analytical method, such as high-performance liquid chromatography (HPLC) or gas chromatography (GC).
The purified sample of 4-(4-aminobenzyl)piperazine-1-carboxylic acid tert-butyl ester is dissolved in a suitable solvent, such as acetonitrile or ethanol, and is then injected into the HPLC or GC system.
The compound is then separated from any impurities and is detected by a suitable detection method, such as UV or mass spectrometry.
The amount of GV-976 present in the sample is then calculated based on the peak area or peak height of the compound.

In conclusion, the production process of GV-976 involves the synthesis of 4-aminobenzyl piperazine and 4-(4-aminobenzyl)piperazine-1-carboxylic acid, followed by the esterification of the carboxylic acid with tert-butyl alcohol.
The final product is then purified and estimated to obtain a pure sample of GV-976.
The production process of GV-976 is a complex and multi-step process that requires the use of various chemicals and reagents, as well as specialized equipment and techniques.
Therefore, it is important to follow proper safety guidelines and to have a thorough understanding of the synthetic route in order to ensure the safe and efficient production of GV-976.