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The main uses and application differences between polyclonal antibodies and monoclonal antibodies

 Last Update: 2022-08-30
 Source: Internet
 Author: User

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Monoclonal Antibody
Monoclonal Antibody Definition: Monoclonal Antibody Production of Monoclonal Antibody Uses:

Detect specific antigens
Detection of individual members of protein families
Create consistent results between experiments/batches
Stain cells with less background - ideal for immunohistochemistry, immunocytochemistry and immunofluorescence experiments
Quantify protein expression (e.
g.
flow cytometry or fluorescence-activated cell sorting)
Detecting changes in molecular conformation
Detection of changes in phosphorylation status
Detecting targets for X-ray crystallography
Create animal models that lack specific cell types
Immunotherapy

Monoclonal antibodies are not suitable for:

Detection of target proteins across species based on homology
Detect low levels of target protein
Detection of conformationally altered proteins

polyclonal	Monoclonal
Heterogeneous antibody population with different paratopes	Homologous population of specific antibodies with one paratope
non-epitope specific	Epitope specificity
Increased likelihood of cross-reactivity with similar antigens	low cross reactivity
Increased likelihood of background noise	Low background noise
batch variability	the same batch
low development cost	high development cost
Fast production (about 3 months)	Slow production (about 6 months)
Many host species to choose from	Few host species to choose from

What are polyclonal antibodies?
Polyclonal Antibody Definition: Use of Polyclonal Antibodies to Produce Polyclonal Antibodies:

Detection of known or unknown antigenic subtypes with high antigenic homology
Detect low levels of specific antigens
Capture as much antigen as possible (e.
g.
immunoprecipitation or chromatin immunoprecipitation)
Detection of denatured proteins
Detection of targets with possible genetic polymorphisms, glycosylation or conformational changes
Detect native proteins across multiple assay types
Detection of targets in solutions of varying pH and salt concentration

Polyclonal antibodies are not suitable for use in the following areas:

Quantitative experiments, such as flow cytometry, can lead to incorrect results due to signal amplification due to multiple binding sites
In case of cross-reactivity problems with highly homologous proteins

Suzhou Alpha Bio

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