The intestinal bacteria are involved in the development of lupus

Zhang Wei to guide the students.
Recently, Zhang Wei, a professor in the department of rheumatology immunology at Concord Hospital in Beijing, published a cover paper in arthritis and rheumatism, the official journal of the American Rheumatology Society, suggesting that the intestinal bacteriologic tract of untreated systemic lupus (hereinafter referred to as lupus) patients has characteristic changes in inflammation and autoimmune disease compared to normal people, the rich species come in part from the mouth, and two bacterial polypeptides can trigger autoimmune response through molecular simulation mechanisms, possibly associated with lupus.
suggests that changes in the gut bacteri group in lupus patients are closely related to immune abnormalities in patients. In the future, we can help treat lupus by regulating the gut bacteriobi. Zhang said.lupus is a typical autoimmune disease characterized by chronic inflammation and multi-organ damage, and the self-tolerance of lupus patients decreases and continuously produces autoantibodies. The complex autoimmune response mechanism during the onset of lupus has not been fully clarified.
in addition to certain genetic and environmental triggers that can cause disease, industry insiders are increasingly concerned about the important regulatory role of gut microbiota in the host immune system. However, there is still no in-depth understanding of the exact description of the bacterial characteristics of lupus patients and related pathogenic mechanisms.
, Zhang's team conducted a study on the lupus gut bacteria. In the trial, they included 117 lupus patients who had not been treated or treated with antibiotics for three months, collected their feces and clinical information, and included 115 healthy people of matching gender and age as a control.
, the researchers collected 52 patients with lupus who had been effectively treated for remission (a significant reduction in disease activity score) for their own back-to-back studies. All the samples collected were tested for intestinal bacteri groups by bird gun macro genome sequencing, and species and functional annotations were made using HUMAnN2, a classical macrogenomic analysis method, and the bacterial relevant analysis was carried out.
Finally, based on a species-level analysis, the researchers found that the diversity of intestinal bacterios in lupus patients decreased significantly, with a more pronounced decrease in lupus nephritis patients; Untreated lupus patients had a significant increase in six types of bacteria, such as Clostridium sp. in their intestines, and a significant decrease after treatment.
" and there were differences in function and control groups in the gut bacteria of lupus patients, and the functional differences were more significant than between species composition. The paper's first author, Dr. Chen Yandi, department of rheumatology immunology at the Concord Medical College in Beijing, said that in untreated lupus patients, the synthetic, inositol-degrading and thiamine (vitamin B1) synthesis pathlines of branched-chain amino acids were significantly reduced and recovered after treatment;
, according to reporters understand that at present, the changes in the intestinal bacteria through the existing clinical test indicators, it may be indirectly detected. Multifactor analysis showed that blood creatinine levels in both lupus patients and healthy people partly explained the individual differences in the overall species composition of the gut bacteria.
, the researchers also found a significant association between abnormal partial intestinal bacterial abundance in lupus patients and some important clinical indicators of lupus. This suggests that the gut bacteria are likely to be involved in the development of lupus, especially in patients with renal function changes.For further study, Zhang's team tested the fecal macrogenome in lupus mice and compared the results with data from lupus patients, and found that associated metabolic abnormalities such as arginine, tryptophan, and branched chain amino acids mediated by intestinal bacteriums had common changes in lupus patients and mice.
" these bacterial paths are likely to be involved in the progress of lupus, is the future autoimmune field bacteria-related functional verification can be explored direction. What's interesting, Zhang said, is that some of the bacteria that significantly increase in the intestines of lupus patients and are "booming" are actually "recidivists."
previous studies have shown that three of the six lupus-rich bacteria are also closely related to the occurrence of inflammation in the mouth. To do this, the researchers included a new team of healthy people, collecting data on their oral saliva macro genomes and performing SNP characteristic analysis of strains.
results suggest that some lupus patients have more intestinally rich strains similar to those found in the oral cavity. In other words, lupus intestinal rich bacteria are likely to come from the mouth.
, the researchers also found direct evidence of intestinal bacteri groups involved in the development of lupus. They compared the genomes of bacteria closely related to lupus with peptide segments that have been proven to be lupus autoantigen progesteres (obtained from the immunosebit database) and used in-body experiments to verify whether these bacterial peptides, which are highly similar to their own antigen progesteres, activate the lymphocytes of lupus patients and produce inflammatory reactions.
"We found that the peptide segments produced by the Odoribacter splanchnicus and Akkermansia muciniphila bacteria were not only highly similar to the supersebits of lupus-characterized autoigens Sm antigens and Fass antigens, but also activated CD4-T cells and B cells, respectively, which may be involved in the development of lupus through molecular simulation mechanisms. Chen said.
For this study, industry experts believe that the study has deepened scholars' understanding of the characteristics of the lupus intestinal bacterios, through large samples and clinical cohort studies without therapeutic intervention, and has also suggested some possible mechanisms for the involvement of abnormal intestinal bacterios in the progress of lupus disease.
future, we will further analyze the characteristics of the intestinal virloids of lupus patients and explore mechanisms to develop bacteriologic-related complementary treatments in the field of autoimmune diseases. Zhang said. (Source: Zhang Siwei, China Science Journal, )
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