The Instruction of 5-METHYLTETRAHYDROFOLIC ACID DISODIUM SALT

5-Methyl-tetrahydrofolate (5-MTHF) is a naturally-occurring folate derivative that plays a crucial role in the metabolism of amino acids and the synthesis of nucleic acids.
Folate is essential for cell division, growth, and development.
However, folate levels can be affected by various factors, including age, genetics, and dietary deficiency.

5-MTHF is converted to tetrahydrofolate (THF) by the enzyme methylenetetrahydrofolate reductase (MTHFR), which is widespread in human tissues.
The THF derivative can then proceed through the folate metabolic pathway, leading to the formation of 10-formylpentanoic acid (10-FPA) and 5,10-methylene-tetrahydrofolic acid (5,10-MTHF).

In the chemical industry, 5-MTHF is used as an intermediate in the synthesis of various compounds, including agrochemicals, pharmaceuticals, and bulk chemicals.
The compound is also used as a nutritional supplement for individuals with folate deficiency or genetic MTHFR mutations.

5-MTHF can be synthesized through various chemical methods, including enzymatic and chemical reductions.
In the enzymatic reduction method, 5-MTHF is synthesized from 5,6-bened Cinchona alkaloid using the enzyme benzene-Δ2-1,2-dioxygenase.
In the chemical reduction method, 5-MTHF is synthesized by reducing 5,6-bened Cinchona alkaloid with lithium aluminum hydride (LiAlH4) or sodium borohydride (NaBH4).

5-MTHF can also be synthesized in a one-step reaction by reacting 7-aminocephalosporanic acid (7-ACA) with formaldehyde in the presence of a strong acid catalyst, such as sulfuric acid, to form 5-MTHF with a high yield.

5-MTHF can be purified using various methods, including crystallization, column chromatography, and high-performance liquid chromatography (HPLC).
Crystallization is a common method for purifying 5-MTHF, as it is often found to be present as crystals in the synthetic process.
Column chromatography and HPLC can also be used to purify 5-MTHF, depending on the specific requirements of the application.

5-MTHF can be used as an intermediate in the synthesis of various compounds, including agrochemicals, pharmaceuticals, and bulk chemicals.
For example, 5-MTHF can be converted to 5-formyl-THF using the enzyme formyltetrahydrofolate synthetase, which is involved in the conversion of 5-MTHF to THF.
THF can then be used in the synthesis of various compounds, including pesticides, pharmaceuticals, and other chemicals.

5-MTHF can also be used as a nutritional supplement for individuals with folate deficiency or genetic MTHFR mutations.
Folate deficiency can lead to birth defects, and genetic MTHFR mutations can affect folate metabolism and lead to folate deficiency.
5-MTHF supplements can help restore folate levels and prevent related health issues.

However, it is important to note that excessive intake of 5-MTHF should be avoided, as high levels of 5-MTHF can lead to toxicity and adverse effects.
It is also important to consult with a healthcare professional before using 5-M