The impact of the laboratory environment on animal safety

In order to ensure the quality of SPF-level laboratory animals, it is necessary to ensure that the barrier facility environment in which they live is safe, so how to control the safety of the barrier facility environment, PATEO Xiaobian takes you to understand the impact of the
laboratory environment on animal safety.

First, the temperature is too low will lead to the postponement of the experimental size rat sex cycle, and the reproductive algebra will decrease
.

Too high a temperature can lead to a decrease in the quality and quantity of male sperm, and female animals will have sexual cycle disorders, decreased lactation ability or refusal to breastfeed, and pregnancy rates will decline
.

Second, relative humidity is closely related
to the body heat regulation of animals.

If the humidity is too high, pathogenic microorganisms are prone to multiply, which can easily cause the occurrence
of certain infectious or non-communicable diseases.

Humidity is too low to lead to dust, humidity below 40% of the rats are prone to ringtail disease, female rats are prone to feeding phenomenon, and rats will have stunted growth
.

Third, the minimum static pressure difference in the communication area ensures the independent environment of each area by controlling the differential pressure gradient between the communication areas, and at the same time avoids the polluted air in the non-clean area from entering the clean area
.

Fourth, the number of air changes and the speed of air flow provide sufficient oxygen and reduce heat stress; It can regulate temperature and humidity, reduce indoor dust and harmful gases, and control the prevalence
of infectious diseases.

The number of ventilation is too small, the air in the feeding room is dirty, the number of ventilation is too much, and the animals are easily dehydrated
.

Fifth, air cleanliness and air cleanliness of air settlement bacteria can control the amount of dust in the environment and particle size, to avoid its impact on
the health of experimental animals and personnel as an allergen and microbial carrier.

Air sedimentation bacteria can directly reflect the degree of contamination of pathogenic microorganisms in the environment through the number of colonies, and indirectly monitor the cleanliness of the air in the environment and the number of
air changes.

Sixth, noise can cause irritating tension in animals, and behavioral and physiological reactions
occur.

Too high noise will lead to a decrease in the average number of litters, the rate of milk ion, the rate of bite killing and the rate of feeding in the rat herd, and the higher the noise value, the more obvious
the phenomenon.

Experiments showed that after 2 minutes of the effect of 10KHz 100dB ringtone on 36-52 days old DBA and C57BL/6J, DBA could spasm and be severely deadly, but C57BL/6J mice did not respond
.

Seventh, illuminance and light and dark time can ensure that animals form a normal physiological cycle, affecting metabolism and reproduction
.

As the illuminance increases, the weaning mortality rate of albino rats increases
.

8.
Increased ammonia concentration can irritate the conjunctiva of the eye, nasal mucosa and respiratory mucosa and cause lacrimation, cough, and severe cases can cause edema and lead to animal death
.

At the same time, it can affect the calving capacity, newborn weight, eclipsing rate, fetal interval and growth
of rats and rats.