The Guangzhou Institute of Health of the Chinese Academy of Sciences reveals a new mechanism for neural stem cell differentiation

recently, Zheng Hui of the Guangzhou Institute of Biomedicine and Health of the Chinese Academy of Sciences and the Guangzhou Laboratory of Regenerative Medicine and Health in Guangdong Province revealed the role of non-opioid subject pathways in regulating neural stem cell differentiation with the opioid-subject antagonist naloxone. The findings were published online
.
neural stem cell (NSCs) differentiation refers to the process by which neural stem cells produce cells such as neurons, asstary glials, and less protrusive glials through asymmetric division. Opioids, as a clinically commonly used analgesiology drug, greatly alleviate the suffering of patients, but the tolerance and addiction process of opioids also significantly affect the ability to learn and remember. Several studies have shown that opioids can regulate the direction of NSCs differentiation, affecting the occurrence of body-forming nerves and their ability to learn and remember, so is the effect of opioids on NSCs differentiation entirely dependent on opioid subjects?
team studied the mechanism by which opioids regulate NSCs differentiation, using the opioid-controlled antagonist naloxone. It was found that in NSCs with very low expression of opioid subjects and their endogenous media, naloxone promotes NSCs differentiation to neurons by entering the cell, while naloxone-iodine methicoxide, a naloxone-iodine oxide, which loses the ability of cell membrane penetration, does not affect the differentiation direction of NSCs. Further studies have shown that naloxone treatment alters the DNA dna of TET1 in cells and the level of DNA methylation in the genome. Subsequently, the team used TAT1 to knock out cells to demonstrate that naloxone's regulatory effect on NSCs differentiation depended on TAT1, independent of TAT2 or TAT3 in the same family. In addition, the team confirmed that other opioids can also regulate NSCs differentiation through non-opioid-subjected pathways, such as adding morphine in the early stages of NSCs differentiation, which can also promote NSCs to neuron differentiation.
in the later stages of neurodifferentiation, where the level of expression of opioids is gradually increasing, morphine promotes differentiation in the direction of astrological glial cells through the opioid-subject approach. This study shows the role of non-opioid-subject pathways in the process of opioid regulation NSCs differentiation, and provides a new understanding of the regulatory role of opioids in the nervous system. (Source: China Science Journal Zhu Hanbin Huang Boquan)
related paper information: