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Recently, the scientific and technological innovation team of the protection and utilization of forage germplasm resources of the Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, found ERF053, a key gene for the regulation of drought resistance in alfalfa, which provides important genetic resources and reference for
further improving the stress tolerance of transgenic plants and carrying out research on forage stress resistance genetic improvement.
The results were published in Frontiers in Plant Science
.
further improving the stress tolerance of transgenic plants and carrying out research on forage stress resistance genetic improvement.
The results were published in Frontiers in Plant Science
.
Alfalfa is an important legume forage with strong drought resistance and can be used as an important genetic source
for forage molecular breeding.
In this study, the researchers simulated drought stress by applying 400 mM mannitol to alfalfa, analyzed the morphological and physiological and biochemical index changes of alfalfa seedlings at different treatment time points, screened candidate genes that were differentially expressed and significantly responded to drought stress, and found that four ERF transcription factor genes were closely related to drought stress, among which MfERF053 gene had the highest
expression level in the roots of alfalfa.
for forage molecular breeding.
In this study, the researchers simulated drought stress by applying 400 mM mannitol to alfalfa, analyzed the morphological and physiological and biochemical index changes of alfalfa seedlings at different treatment time points, screened candidate genes that were differentially expressed and significantly responded to drought stress, and found that four ERF transcription factor genes were closely related to drought stress, among which MfERF053 gene had the highest
expression level in the roots of alfalfa.
The results showed that when MfERF053 was overexpressed in the model plant Arabidopsis, the root length, lateral root number and fresh weight of transgenic plants were significantly better than wild-type controls
under drought, salt stress and exogenous abscisic acid treatment.
Plants overexpressing the MfERF053 gene also had significantly better growth conditions under drought and salt stress than wild types
when grown in soil.
At present, this gene has also been overexpressed in alfalfa, which can be used to further elucidate its regulatory mechanism and apply it to the improvement
of stress resistance traits in alfalfa.
under drought, salt stress and exogenous abscisic acid treatment.
Plants overexpressing the MfERF053 gene also had significantly better growth conditions under drought and salt stress than wild types
when grown in soil.
At present, this gene has also been overexpressed in alfalfa, which can be used to further elucidate its regulatory mechanism and apply it to the improvement
of stress resistance traits in alfalfa.
This research was supported
by the National Natural Science Foundation of China and the National Modern Agricultural Industry Technology System Project of the Ministry of Agriculture and Rural Affairs.
by the National Natural Science Foundation of China and the National Modern Agricultural Industry Technology System Project of the Ministry of Agriculture and Rural Affairs.
Original link:
