The first "protein map" of healthy human heart

Protein is the molecular machine of cell, which is made by DNA storage code The change of DNA or protein level is likely to lead to disease The change of molecular level is considered as the root cause of heart disease, so it is very important to know the type, distribution and function of protein accurately for heart research Heart protein mapping scientists identify protein information in all areas of the heart, such as the heart valve, the heart cavity, and major blood vessels In addition, they studied the protein composition of three different types of heart cells: cardiac fibroblasts, smooth muscle cells and endothelial cells Through mass spectrometry, they found nearly 11000 different proteins Previous studies have focused on single cell types or pathological heart tissue "There are two problems," says Sophia doll of mpib "First, the results do not give a full picture of all regions and tissues of the heart; second, there is a lack of data on healthy hearts Our research eliminates these two problems and provides reference for future research " "From this map of human heart proteins, we found that all healthy hearts work in very similar ways," Sophia doll said "We measured the protein components of all heart tissues, but they didn't differ much What's more, we were surprised to find that, although the functions of the left and right hearts were quite different: the right half carried oxygen deficient blood and the left half carried oxygen rich blood, their protein composition was very similar " Difference between morbid and healthy to test whether healthy heart data can guide disease detection, the researchers compared the cardiac proteome of patients with atrial fibrillation Results the preliminary clue of the cause of the disease was found: the protein responsible for providing energy to cells in the diseased heart tissue was different from that in the normal heart Another interesting finding is that although the energy metabolism related proteins have changed in all patients, the individual changes are different These findings suggest the importance of personalized care "Although all patients have similar symptoms, the data tell us that each case has different molecular dysfunction, and we need to learn how to recognize and treat individual differences in heart drugs," said Dr Markus Krane, deputy director of cardiovascular surgery, German heart center In less than two days, Dr Markus Krane and his colleagues in cardiovascular surgery collected more than 150 tissue samples from 60 cardiac and forensic samples Using fine cell culture methods, they extract all kinds of cells from it A large number of heart samples is a key factor in the study of cardiac region refinement Professor Matthias Mann, head of the mpib proteomics and signal transduction team, and his team conducted extensive mass spectrometry of all samples The progress of mass spectrometry and sample processing technology has opened up the way for personalized medical treatment The researchers have improved the measurement technology in terms of accuracy, repeatability and rapidity The result is that the protein detection of the whole heart area can be completed in less than 2 days, and the original detection speed has been increased by 2 times.