The first case of cloning tumor mutation gene and replicating individual polypeptide for tumor treatment

Recently, the world's first clinical application of cloned tumor mutation gene replication personalized polypeptide in cancer treatment technology has been successful in Tianjin Beichen hospital Dr Du Xueming, director of Oncology Department of Tianjin Beichen hospital, and Professor Gregory lizee of MD Anderson Cancer Center, a well-known Cancer Research Institute in the United States, jointly published rapid tumor expression in an Asian lung cancer patient following personalized Neo epiope in the famous American Medical Journal oncology Peptide, a medical paper, elaborates how to use the gene mutation information of cancer patients to develop personalized tumor clone peptide vaccine, and apply it to patients Finally, by activating the patients' autoimmune system, it can effectively inhibit the proliferation of cancer cells and prolong the life of patients The success of this clinical application also marks that China has been in the forefront of the world in the development of personalized tumor clone peptide vaccine 　　 Cancer has seriously affected the national health index According to the data from the National Cancer Center, about 10 thousand people are diagnosed with cancer every day in China, and lung cancer ranks first among the incidence rate and mortality rate For a long time, the diagnosis and treatment of lung cancer are mainly based on the histological type Diagnosis and treatment of early cancer patients, for local, surgery and radiotherapy combined treatment or radiotherapy and chemotherapy However, due to the concealment of lung cancer, most of the patients are in the late stage when they are diagnosed, so they can't be treated by surgery, only chemotherapy alone However, chemotherapy is toxic and causes serious injury to the patient's body, and the median survival time is only 12 months Chemotherapy is not the best treatment for patients In recent years, with the progress of precision medical technology, for some patients who are suitable for targeted treatment, the molecular targeted drug treatment mode is adopted to improve the survival period; however, in the effective targeted treatment patients, half of the patients will still have drug resistance phenomenon in about 10 months In the long-term clinical practice, doctors and patients have been looking forward to more effective new drugs and new technologies to help more cancer patients Cloning tumor mutation gene and replicating individual polypeptide for tumor therapy In 2013, director Du Xueming and Professor Gregory lizee jointly conducted an experiment to clone the mutant gene of lung cancer patients In the process of the experiment, we cloned the mutant gene of lung cancer patients, combined with HLA typing of patients, carried out affinity matching analysis, and copied the personalized peptide which can be combined with T cells When these small protein fragments carrying gene mutation information combine with T cells in patients, the human body will produce T cells that specifically kill tumors After one month's treatment, the tumor of lung cancer patients who had no medicine was gradually reduced or even disappeared The blood sample data of different stages of diagnosis and treatment showed that the specific T cells in the patients multiplied in large numbers, and with the disappearance of the tumor, the T cells gradually decreased Professor Gregory lizee explained, "generally, there are about 3-5 individual peptides that can be cloned out of 100 mutant genes Many of them only exist in tumor cells of a single patient Because they don't exist in healthy cells, they are called "new antigens." Individual polypeptides are cloned in the laboratory, and adjuvants are added to strengthen the role of the immune system, so as to kill cancer cells In fact, such spontaneous remission also reflects the real biological phenomenon that the specific activation of the immune system can control tumors This allowed biologists to open up other avenues for the immune system to attack only antigens found in cancer cells, usually in the cancer cells of a patient Professor Bai Li, Department of oncology, General Hospital of the people's Liberation Army, said: "tumor itself is a genetic disease, and the occurrence of tumor without gene change is relatively rare According to some of its characteristics, based on genetic or genomic changes for analysis, targeted selection of some drugs to achieve the same disease different treatment, different disease treatment, to achieve the purpose of a precise treatment The incidence of asthma, diabetes, arthritis, Alzheimer's disease and tumor are relatively high in the population, and the heterogeneity of tumor is the strongest A drug can be about effective, covering one-third of patients is good, so it shows the importance of individualized treatment The study showed that after individualized analysis, the effective rate reached 31%, and the patients without screening was 10% " "It is well known that the high carcinogenic effect of tobacco smoke makes lung cancer one of the most variable cancers of all cancers," said Du In Asia, the high incidence of L858R mutation and the combination of peptide HLA with alloimmunoglobulin indicate that targeted immunotherapy based on new epitopes of CD8 + cytotoxic T cells (CTLs) will benefit 5% of Asian lung cancer patients For the cancer patients with high incidence in China, the treatment of individualized cloned polypeptide can provide a new treatment method and means for the cancer patients with high incidence in China " Personal customized cancer treatment plan is about to be realized Although the clinical trials of tumor vaccine have been carried out for more than 20 years, the immune response of the subjects to the vaccine has not been ideal One of the main features of this method, which is different from most of the previous tumor vaccines, is to obtain the mutation features related to the pathogenic genes of patients based on the next generation gene sequencing technology According to the results of the whole exome sequencing of tumor tissue and the detection results of specific panel genes, then predict the mutant peptide antigen of patients through CWES, combine the individual HLA typing, and customize the individual mutant peptide immune epidemic Miao to achieve From the current research conclusion, this therapy is suitable for solid tumor patients with multiple gene mutations and drug resistance "It's impossible for any two patients to have the same cancer cell specific new antigen," said Dr Catherine Wu of Dana Farber Institute in Boston "That's why we have the opportunity to truly personalize cancer treatment by assembling new antigens from specific patients." Lung cancer vaccine will help Chinese patients improve their health index In recent years, national policies are also encouraging the scientific treatment of cancer, extending the lives of patients and improving the health of the whole people The Central Committee of the Communist Party of China and the State Council issued the outline of "healthy China 2030" plan, which is the first time for China to put forward a medium and long-term strategic plan in the field of health at the national level It is also an important embodiment of China's active participation in global health management and the first time to raise health to the height of national strategy According to the outline, "in 2030, the health management of chronic diseases in the whole population and life cycle will be realized, and the overall 5-year survival rate of cancer will be increased by 15%" In the "notice on strengthening the standardized diagnosis and treatment management of tumor", the national health and Family Planning Commission clearly requires "optimizing the diagnosis and treatment mode of tumor, that is, implementing the" single disease, multi-disciplinary "diagnosis and treatment mode." As Beichen hospital in Tianjin fills in the blank of individual clone peptide immunotherapy for cancer, and as the project further improves the clinical application test information, it will help more cancer patients to prolong their lives.