The effect of Ginkgo biloba extract on the adipogenic differentiation of rat bone marrow mesenchymal stem cells

Recently, researchers from the Department of Prosthodontics, Stomatology Hospital, Jilin University published a paper to explore the effect of Ginkgo biloba extract (GBE) on the adipogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) in rats, and to clarify the significance of GBE in the treatment and prevention of osteoporosis at the cellular level The results showed that GBE could inhibit the adipogenic differentiation of BMMSCs in vitro, and the inhibition increased with the increase of GBE concentration This article was published in the 2nd issue of Journal of Jilin University (Medical Edition) in 2015 50, 100, 150, 200 and 400mg · L-1 GBE and lipogenic inducer were added to the purified BMMSCs of the third generation (50, 100, 150, 200 and 400mg · L-1 In GBE group, BMMSCs without drugs were cultured for 7 days, and the morphology and expression of surface antigens CD44, CD105 and CD34 were observed; the lipid droplet formation in BMMSCs was observed by oil red O staining, and the lipogenic capacity of BMMSCs was quantitatively analyzed; the fatty acid binding protein (ap2) and peroxisome proliferator activated receptor (PPAR) in BMMSCs were detected by RT-PCR The expression level of PPAR - γ mRNA Morphological examination, primary BMMSCs cultured in vitro After 3 days, the cells showed fusiform shape, and after 7 days, the cells showed whirlpool like growth and arranged in the same direction; surface antigen marker staining showed positive expression of CD44 and CD105, while CD34 showed negative expression, indicating BMMSCs; oil red O staining showed that a large number of lipid droplets formed under microscope about 7 days later, and the number of lipid droplets decreased with the increase of GBE concentration; compared with the control group, the A510 value of BMMSCs in other groups decreased (P < 0.05) Compared with the control group, the expression of AP2 and PPAR - γ mRNA in other groups decreased (P < 0.05).