The difference between high speed centrifuge and low speed centrifuge

Centrifuges, according to the size of the workload, are mainly selected from two aspects: speed and capacity
.

The following is a detailed introduction to some common sense of centrifuges: (1) Speed: centrifuges are divided into low-speed centrifuges (<10000 rpm/min) and high-speed centrifuges (10000 rpm/min-30000 rpm/ min), ultra-high-speed centrifuges (>30000 rpm/min), each centrifuge has a rated maximum speed, the maximum speed refers to the speed under no-load conditions, but the maximum speed depends on the type of rotor , The size of the sample quality varies
.

For example, the rated speed of a centrifuge is 16,000 rpm/min, which means that the rotor rotates 16,000 times per minute at no load.
After adding the sample, the speed will definitely be less than 16,000 rpm/min
.

Different rotors have different maximum speed (one imported centrifuge can be equipped with multiple rotors), the horizontal rotor can reach 15000 rpm/min, but the angle rotor can reach about 14000 rpm/min, the specific difference should be consulted in detail Product sales personnel and relevant technical personnel of the production plant, so be careful in the choice of rotation speed, and the maximum rotation speed of the selected centrifuge should be higher than the target rotation speed
.

For example: the target speed is 16000 rpm/mIn, the maximum speed of the selected centrifuge must be higher than 16000 rpm/min
.

(2) Temperature: Some samples (such as proteins, cells, etc.
) will be destroyed in a high temperature environment, so a refrigerated centrifuge should be selected, and the refrigerated centrifuge has a rated temperature range
.

When the centrifuge is running at high speed, the heat generated by the centrifuge is balanced with the cooling system of the centrifuge at a certain temperature (generally, the sample for refrigerated centrifugation needs to be kept at 3°C ​​to 8°C).
The specific amount that can be achieved is also related to the rotor.
The rated temperature range is -10°C to 60°C.
When the horizontal rotor is installed, it can reach about 3°C.
If it is an angle rotor, it may only reach about 7°C.
This point should also be consulted in detail with the product sales staff and the relevant technical staff of the production plant
.

(3) Capacity: How many sample tubes need to be centrifuged each time 7 How much capacity is required for each sample tube 7 These factors determine the total capacity of a centrifuge.
Simply put, the total capacity of the centrifuge = the capacity of each centrifuge tube × centrifugation The number of tubes, the total capacity and the size of the workload are matched
.

(4) Rotor: The rotor of the centrifuge is mainly divided into two types, horizontal rotor: the hanging blue is in a horizontal state during operation, at a right angle to the rotating shaft, and the sample concentrates the sediment at the bottom of the centrifuge tube: Angle rotor: the centrifuge container and the rotating shaft form a fixed angle, the sample concentrates the sediment at the bottom of the centrifuge tube and the side walls near the bottom
.

Choose a horizontal rotor if you want the separated sample to be concentrated at the bottom of the centrifuge tube, or choose an angle rotor if you want the sample to be concentrated at the bottom of the centrifuge tube and on the side walls near the bottom
.

There are also some special experiments or special samples that require special rotors, such as: large-capacity baskets (mostly used in blood banks), microplate rotors, slide rotors, PCR rotors, test tube rack rotors and capillary rotors
.

Rotors have fixed specifications, which are combined with the capacity of the centrifuge, such as a 36 × 5 ml angle rotor, which determines both the type of rotor and the capacity of the centrifuge, so the choice of rotor is very important
.

(5) Control system: The centrifuges all use microcomputer control systems, which can not only ensure the safe operation of the centrifuges, but also automatically complete the work tasks
.

Now many centrifuges have better humanized control systems.
For example: rotor identification function, safety lock function, fault prompt function, acceleration and deceleration curve and so on
.

In addition to the above points, pay attention to some details and necessary accessories
.

The main component of the centrifuge is the motor.
The motor is divided into a motor with a carbon brush and a motor without a carbon brush.
The former has been eliminated.
Most of the current centrifuges are brushless motors, and some motors also have a braking function
.

Refrigerated centrifuges are also different in terms of refrigeration.
Now, of course, the environmentally friendly technology is fluorine-free refrigeration
.

In addition, the noise problem should be considered, and a centrifuge with less noise should be selected as much as possible, so as to maintain a comfortable experimental environment
.

Be cautious about accessories.
Some experiments use special centrifuge tubes (centrifugation of toxic samples or samples that require ultra-high-speed centrifugation).
Such centrifuge tubes must be equipped with corresponding tube sleeves to be safer
.

There are also some special sample containers (irregular sample bottles, blood bags, etc.
), these details and accessories must be carefully considered when choosing a centrifuge, otherwise it will not be able to work normally