The difference between biosafety cabinet, ventilation cabinet and super clean bench

Biosafety cabinet, fume hood and super clean working table are common operation equipment in food laboratory Although they seem to have similar structure and appearance on the surface, they are actually three types of equipment with different functions Therefore, it is necessary to clearly understand the distinction between biosafety cabinet, fume hood and super clean working table in laboratory construction First of all, we should make it clear that the fume hood and super clean bench are not biosafety cabinets and cannot be used in the experiment or production process involving infectious pathogenic microorganism materials The biological safety cabinets (BSCs) are designed to protect workers, laboratory environment and the safety of experimental materials, such as bacterial strains and diagnostic specimens, so as to avoid exposure to infectious aerosols and spills that may occur during the above operations The manual of laboratory biosafety published by the World Health Organization (who) clearly states that "biosafety cabinet can effectively reduce laboratory infection and cross contamination of culture caused by aerosol exposure, while biosafety cabinet can also protect the working environment" Of course, the premise of effective work of safe cabinet is to choose qualified safe cabinet and use safe cabinet correctly The current effective national standard general principles for microbiological examination of food (GB 4789.1-2010) stipulates that the isolation and identification of pathogenic microorganisms should be carried out in the secondary biosafety laboratory (BSL-2), while the general requirements for laboratory biosafety (GB 1948-2008) also makes clear requirements for biosafety cabinets in the definition of the secondary biosafety laboratory Therefore, the biosafety cabinet has become one of the necessary equipment to carry out the inspection of food pathogenic microorganisms The fume hood (fume hood) is designed to remove corrosive chemical gases and toxic fumes during chemical experiments As there is no HEPA filter, the fume hood can not effectively remove the microbial media Microbial samples placed in the fume hood will be spread outside the fume hood, polluting the laboratory environment Ultra clean bench (ultra clean bench) is designed to protect the test article or product It can prevent the test article or product from being polluted by dust or bacteria outside the work area by blowing the vertical or horizontal laminar air of the work area Once the samples of infectious pathogenic microorganisms are placed in the work area, the laminar air will blow the air with such microbial medium to the front desk staff, resulting in danger Therefore, it is suitable for the inoculation and separation of non pathogenic microorganisms The biosafety cabinet is designed to protect the working personnel, laboratory environment and experimental products from being exposed to the infectious aerosols and splashes that may be generated in the above operation process when operating the infectious experimental materials such as primary culture, bacterial strain and diagnostic specimen Its purpose is to protect the experimental process, operators and the environment from harm The fume hood and super clean bench are not biosafety cabinets and cannot be used in the experiment or production process involving microbial materials The selection of the biosafety cabinet Zui basically depends on the type of test article used in the biological test: 1 If the biological test involves microbial samples and trace chemical reagents (non corrosive), it is necessary to use the safety cabinet equipped with the discharge pipeline Because HEPA filter can not effectively filter and remove chemical gases, if it can not be discharged, it will pose a threat to the laboratory staff and the environment; 2 If cytotoxin drugs are involved in biological tests, such as all kinds of cancer treatment drugs, the cytotoxin safety cabinet must be used 3 If only microbiological samples are involved in the biological test, the basic type of biosafety cabinet can be used; in addition, the distinction between biosafety cabinet and ventilation cabinet / ultra clean bench needs to be clearly understood The fume hood and super clean bench are not biosafety cabinets and cannot be used in the experiment or production process involving microbial materials Ultra clean bench (ultra clean bench) is designed to protect the test article or product It can prevent the test article or product from being polluted by dust or bacteria outside the work area by blowing the vertical or horizontal laminar air of the work area Once the microbial sample is placed in the working area, the laminar air will blow the air with microbial medium to the front desk staff, which will cause danger The fume hood (fume hood) is designed to remove corrosive chemical gases and toxic fumes during chemical experiments As there is no HEPA filter, the fume hood can not effectively remove the microbial media Microbial samples placed in the fume hood will be spread outside the fume hood, polluting the laboratory environment.