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The name(i)Tritone Reaction α-
amino acids
and hydratedthone (phenylacone tritone hydrocarbons), the blue reaction, because the
protein
is composed of many α-amino acids, so also present this color reaction.(ii) Biuret Reactionprotein is purple-red with copper sulfate in an alkaline solution, known as biresorptional reaction. This reaction occurs in
compounds
, which contain more than two -CO-NH-bonds in the molecule, and the amino acids in the protein molecules are connected by peptide bonds, so that all proteins can react with
reagents
of the double cocoon.(iii) Millon ReactionProtein solution with Miron reagents (mercury nitrite, mercury nitrate and nitric acid mix), protein precipitation first,
heating
then red precipitation, which is unique to the phenolic nuclei of tyrosine, so proteins containing tyrosine are Mellen reaction.addition, protein solutions can also react with phenol reagents, acetaldehyde acid reagents, thick nitric acid, etc.
