The Chinese Academy of Sciences has teamed up with the City University of Hong Kong to develop microflow control chips for gastric cancer detection.

The separation and symptomatics of cancer cells are essential for early diagnosis of peritoneal metastasis.
however, due to the low content of cancer cells in the patient's abdominal irrigation fluid, the sensitivity of the traditional detection method is low, which can not meet the clinical needs.
Recently, researchers from the Shenyang Institute of Automation (SIA) of the Chinese Academy of Sciences and CityU of Hong Kong developed a photo-induced electrodynamics (OEK) microflectority and microflation of gastric cancer cells, which is highly effective and sensitive to rapidly detect peritoneal metastasis and obtain the electrical properties of cells.
Their findings, published August 5 in the journal Science Advances, are entitled "Detected and isolation of free cancer cells from ascites and peritoneal lavages using the optically induced electrokinetics (OEK)" in clinical practice, where cytological examinations of ascites and peritoneal irrigation are usually performed to detect free cancer cells in the peritoneal cavity.
, however, it is difficult for pathologists to separate stomach cancer cells from mesotheatrial cells (the main components of peritoneal and peritoneal fluid).
, cytological examination relies on cell staining, which is time-consuming and kills cells.
, there is an urgent need for a new, unlabeled, rapid diagnostic method to identify and isolate living stomach cancer cells with high sensitivity.
in the study, researchers developed a new microflow-controlled chip called photo-induced electrodynamics (OEK) that isolates live stomach cancer cells from the patient's abdominal water and characterifies their electrical properties.
cell polymerization model and cell membrane capacitive solution model were established.
OEK microflector control chip is used to detect significant differences in size and electrical characteristics between peritoneal metastasis and cell membrane capacitive gastric cancer cells and abdominal cavity irrigation cells.
therefore, OEK microflow control chips theoretically separate stomach cancer cells from asymptobas and peritoneal lotions.
of the (A) OEK system.
The system consists of a computer, a projector, a spotlight, a signal generator, a three-dimensional (3D) motion table, a charge-coupled device (CCD) camera, and an OEK microflow chip.
of the extraction device in the first part (B).
plastic hose connected to the injection pump is used to collect isolated cells.
structure of the OEK OEK microflow chip.
the chip consists of three parts: glass coated with tin oxide film (ITO glass), used to make microchannel duplex tape and coated hydrogenated amorphous silicon (a-Si:H) ITO glass.
further experiments, the researchers successfully isolated stomach cancer cells from the abdominal water of six patients, with a purity of up to 71%.
compared with the traditional clinical peritoneal transfer detection method, this new method solves the problem of low sensitivity.
is also a labelless, non-destructive and fast technology that allows researchers to isolate and collect stomach cancer cells from OEK microflow-controlled chips in less than five minutes.
they also obtained cell membrane capacitors for stomach cancer cells and peritoneal irrigation cells.
data can be used as biomarkers for cell marker information. The results of
cell polarization model and cell separation show that OEK microflector control chip is capable of detecting free cancer cells in peritoneal water, which has the advantages of fast speed, simple operation and unmarked, and can accelerate the diagnosis of gastric cancer of peritoneal metastasis.
addition, this is the first time OEK has been successfully applied to the separation of clinical gastric cancer asservage samples.
: 'Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)' Science Advances (2020). DOI: 10.1126/sciadv.aba96 !-- !--28