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Tepary Bean (Phaseolus acutifolius)

 Last Update: 2020-11-17
 Source: Internet
 Author: User

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Phaseolus beans are among the major legumes for food consumption, especially in Latin America, Africa, and Asia. Tepary bean (
Phaseolus acutifolius
L. Gray) is one of the five cultivated species of the genus Phaseolus. This chapter describes an
Agrobacterium
-mediated transformation protocol for P. acutifolius based on cocultivation of callus, derived from cotyledonary nodes, with
Agrobacterium
. The selectable marker gene used is neomycin phosphotransferase II (
nptII
), and the selection agent is geneticin. Selection of transgenic callus material is achieved through four to five passages on geneticin-containing medium, after which shoots are induced on medium without selection agent. The protocol as described here has been applied to transform a cultivated variety of P. acutifolius, TB1, and also with some modifications to a wild genotype, NI576 and another cultivated variety, PI440795.

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