Teacher Wang's new problem

Carbon dioxide incubators are widely used in the research and production of medicine, immunology, genetics, microorganisms, agricultural sciences, and pharmacology, and are important basic equipment
for cell and tissue culture research.
It mimics the in vivo microenvironment in vitro for cell culture and tissue construction
.
How to choose the right incubator There are many types and models of CO2 incubators on the market, so how to choose a CO2 incubator suitable for your laboratory? Recently, Mr.
Wang also has the same distress, his laboratory area is relatively compact, and there are more people, and the incubator will be opened many times to pick up and place things during use; HEPA filters
are also required.
If you and Ms.
Wang have the same concerns, you need to consider the following factors: Space planning: Horizontal placement VS vertical stacking CO2 incubator operating modes are horizontal and vertical, which can be placed on the bench or on
a movable base.
Considering the increasing demand for instruments in Mr.
Wang's laboratory in the future, in order to maximize the utilization of laboratory space, the carbon dioxide incubator preferentially chooses one, two or three layers of superimposed combination, and provides a large use space
with a small footprint.
Temperature Control: During the incubation of air-jacketed heating vs.
water-jacketed heating of cells, a constant temperature can greatly reduce the influence of
the environment on the growth status of the sample.
There are two main heating methods for maintaining a constant temperature in the incubator: gas jacket heating and water jacket heating
.
Water jacket heating wraps the inside of the tank through separate hot water intervals on the jacket to maintain a constant
temperature.
The hot water flows through natural convection, circulating continuously at intervals inside the tank, conducting heat to keep the temperature constant
.
As a good thermal insulation substance, water can keep the temperature in the chamber stable for a long time when the instrument is powered off, and its maintenance time is even 4~5 times
that of the gas sleeve heating incubator.
If your experimental environment is not stable (such as power limitations, or frequent power outages) and you need to maintain stable incubation conditions for a long time, then a water-jacketed CO2 incubator is the way to go
.
The gas jacket heating system heats the gas in the box directly through a heater in the
box.
The structure is simpler than that of the water jacket type, and it is often equipped with a high-temperature sterilization system
.
Under the premise of stable power supply in the laboratory, it is recommended that Mr.
Wang purchase an air-sleeved incubator
.
Especially when multiple people share the same incubator or the door needs to be opened and closed frequently due to experimental needs, the gas-sleeved incubator has better temperature stability
.
Equipped with a high-temperature dry heat sterilization system, the chamber can be sterilized regularly to maintain a cleaner culture environment
.
Sterilization method: dry heat sterilization VS moist heat sterilization incubator common thermodynamic sterilization methods include dry heat sterilization and moist heat sterilization
.
Dry heat sterilization heats the box to more than 120 °C, humidity is within 20%, lasts for a certain time, and inactivates proteins and nucleic acids through high temperature to achieve the purpose of
killing.
Dry heat 140 °C, 2-4 hours, is an ideal sterilization method, can play a role in complete sterilization
.
Moist heat sterilization Moist heat sterilization refers to the method of sterilization with saturated water vapor, boiling water or circulating steam, due to the large latent heat of steam, strong penetrating power, easy to denature or coagulate proteins, and eventually lead to the death
of microorganisms.
This is an automated sterilization procedure that effectively removes bacteria, mold, fungal spores and mycoplasma
.
According to the above analysis, Mr.
Wang prefers to choose the Thermo Flying Air Sleeve CO2 Incubator 371
within the budget range.
This incubator adopts an air-sleeved method to maintain a constant temperature, and is equipped with an ISO-5 in-chamber HEPA airflow circulation system and 140°C dry heat sterilization as standard, and can be directly superimposed on
multiple units 。 184.
1 liters electropolished stainless steel box, all components are made of high temperature resistant materials, and its sterilization process does not have any damage to the incubator; ISO-5 intraluminal HEPA airflow circulation system, 24-hour continuous sterilization: filter the air in the box once every minute to ensure that the air in the box is Class 100 superior * gas; Using a special six-sided heating system, the heating element is located on the outer wall of the incubator, directly heated on six sides, compared with the single-point heating method of other brands of incubators, it has better temperature uniformity and shortens the heating and sterilization time; The use of 140 °C dry heat high temperature sterilization mode, can completely eliminate bacteria, fungi, spores, viruses and other microorganisms without secondary pollution; The microprocessor control system is used to monitor the whole process of high-temperature sterilization in real time, and display various status information at different stages of the autoclaving process in real time, including prompting to replace the HEPA filter and remove the sensor
.
Mr.
Wang's problem was successfully cleared, if you need to consult products, please contact Guangzhou Dongrui Technology Co.
, Ltd.