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Tall Fescue (Festuca arundinacea Schreb.)

 Last Update: 2021-01-14
 Source: Internet
 Author: User

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Tall fescue (
Festuca arundinacea
Schreb.) is the predominant cool-season perennial grass in the United States. It is widely used for both forage and turf purposes. This chapter describes a protocol that allows for the generation of large number of transgenic tall fescue plants by
Agrobacterium
-mediated transformation. Embryogenic calli induced from caryopsis are used as explants for inoculation with
A. tumefaciens
. The
Agrobacterium
strain used is EHA105. Hygromycin phosphotransferase gene (
hph
) is used as the selectable marker and hygromycin is used as the selection agent. Calli resistant to hygromycin are obtained after 4–6 wk of selection. Soil-grown tall fescue plants can be regenerated 4–5 mo after
Agrobacterium
-mediated transformation.

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